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Tetrandrine inhibits cell migration and invasion in human nasopharyngeal carcinoma NPC‐TW 039 cells through inhibiting MAPK and RhoA signaling pathways
- Source :
- Journal of Food Biochemistry. 44
- Publication Year :
- 2020
- Publisher :
- Hindawi Limited, 2020.
-
Abstract
- The objective of this study was to investigate the effects of tetrandrine (TET) on cell migration and invasion of nasopharyngeal carcinoma NPC-TW 039 cells in vitro. TET at 1-10 μM did not change cell morphology and also did not decrease the total cell viability and proliferation in NPC-TW 039 cells. It decreased the cell mobility based on decreased wound closure in NPC-TW 039 cells by wound healing assay. TET suppressed the cell migration and invasion using transwell system. TET reduced MMP-2 activities at 1-10 μM and these effects are in dose-dependently. After exposed to various treatments, TET decreased the levels of p-ERK, p-JNK, p-p38, RhoA, and NF-κB at 48 hr. Based on these findings, we may suggest TET-inhibited cell migration and invasion of NPC-TW 039 cells via the suppression of MAPK and RhoA signaling pathways for inhibiting the MMP-2 and -9 expression in vitro. PRACTICAL APPLICATIONS: Tetrandrine (TET), a bis-benzylisoquinoline alkaloid, is obtained from the dried root of Stephania tetrandra. TET has been shown to induce cancer cell apoptosis on human cancer cells but its anti-metastasis effect on cell migration and invasion of nasopharyngeal carcinoma cells has not been investigated. Our results showed that TET significantly repressed the cell mobility, migration, and invasion of NPC-TW 039 cells in vitro that involved in inhibiting RhoA, Ras accompanying with p38/MAPK signaling pathway. We conclude that TET may be the anticancer agents for nasopharyngeal carcinoma therapy in the future.
- Subjects :
- Pharmacology
MAPK/ERK pathway
0303 health sciences
RHOA
biology
030309 nutrition & dietetics
Chemistry
p38 mitogen-activated protein kinases
Biophysics
Cell migration
04 agricultural and veterinary sciences
Cell Biology
medicine.disease
Cell morphology
040401 food science
Tetrandrine
03 medical and health sciences
chemistry.chemical_compound
0404 agricultural biotechnology
Nasopharyngeal carcinoma
biology.protein
Cancer research
medicine
Signal transduction
Food Science
Subjects
Details
- ISSN :
- 17454514 and 01458884
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Food Biochemistry
- Accession number :
- edsair.doi.dedup.....b514cd9674c506a751969747ff765773