Back to Search
Start Over
The effect of diet and radiation on the bacterial symbiome of the melon fly, Zeugodacus cucurbitae (Coquillett)
- Source :
- BMC Biotechnology, Vol 19, Iss S2, Pp 1-12 (2019), BMC Biotechnology
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Background Symbiotic bacteria contribute to a multitude of important biological functions such as nutrition and reproduction and affect multiple physiological factors like fitness and longevity in their insect hosts. The melon fly, Zeugodacus cucurbitae (Coquillett), is an important agricultural pest that affects a variety of cultivated plants belonging mostly to the Cucurbitaceae family. It is considered invasive and widespread in many parts of the world. Several approaches are currently being considered for the management of its populations including the environmentally friendly and effective sterile insect technique (SIT), as a component of an integrated pest management (IPM) strategy. In the present study, we examined the effect of diet and radiation on the bacterial symbiome of Z. cucurbitae flies with the use of Next Generation Sequencing technologies. Results Melon flies were reared on two diets at the larval stage, an artificial bran-based diet and on sweet gourd, which affected significantly the development of the bacterial profiles. Significant differentiation was also observed based on gender. The effect of radiation was mostly diet dependent, with irradiated melon flies reared on the bran diet exhibiting a significant reduction in species diversity and richness compared to their non-irradiated controls. Changes in the bacterial symbiome of the irradiated melon flies included a drastic reduction in the number of sequences affiliated with members of Citrobacter, Raoultella, and Enterobacteriaceae. At the same time, an increase was observed for members of Enterobacter, Providencia and Morganella. Interestingly, the irradiated male melon flies reared on sweet gourd showed a clear differentiation compared to their non-irradiated controls, namely a significant reduction in species richness and minor differences in the relative abundance for members of Enterobacter and Providencia. Conclusions The two diets in conjunction with the irradiation affected significantly the formation of the bacterial symbiome. Melon flies reared on the bran-based artificial diet displayed significant changes in the bacterial symbiome upon irradiation, in all aspects, including species richness, diversity and composition. When reared on sweet gourd, significant changes occurred to male samples due to radiation, only in terms of species richness.
- Subjects :
- Male
0106 biological sciences
Integrated pest management
Melon
lcsh:Biotechnology
Insect Control
01 natural sciences
03 medical and health sciences
Sterile insect technique
Next generation sequencing
lcsh:TP248.13-248.65
Animals
Symbiosis
Phylogeny
030304 developmental biology
Melon fly
0303 health sciences
Bacteria
biology
Research
Tephritidae
fungi
High-Throughput Nucleotide Sequencing
food and beverages
Species diversity
Sequence Analysis, DNA
biology.organism_classification
Animal Feed
Cucurbitaceae
SIT
010602 entomology
Horticulture
NGS
Gourd
Female
16S rRNA gene
Species richness
Biotechnology
Subjects
Details
- ISSN :
- 14726750
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- BMC Biotechnology
- Accession number :
- edsair.doi.dedup.....b50e5a2d8a18df44516c2d0ff84ba126