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Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin's orexigenic action vs. its role as a stress signal are anatomically dissociated.<br />Instituto Multidisciplinario de Biología Celular<br />Laboratorio de Análisis de Imágenes
- Subjects :
- Male
0301 basic medicine
OREXIGENIC
STRESS
Corticotropin-Releasing Hormone
Biología
Endocrinology, Diabetes and Metabolism
Receptor expression
GABA Antagonists
Mice
Corticotropin-releasing hormone
0302 clinical medicine
Endocrinology
Neuropeptide Y
Receptors, Ghrelin
gamma-Aminobutyric Acid
Neurons
Muscimol
Chemistry
digestive, oral, and skin physiology
purl.org/becyt/ford/3.1 [https]
Neuropeptide Y receptor
Ghrelin
Medicina Básica
Psychiatry and Mental health
Infusions, Intraventricular
Gene Knockdown Techniques
GABAergic
purl.org/becyt/ford/3 [https]
hormones, hormone substitutes, and hormone antagonists
medicine.drug
endocrine system
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
NEUROPEPTIDE Y (NPY)
Inmunología
Orexigenic
Arginine
Stress
Article
gamma-Aminobutyric acid
03 medical and health sciences
Neuropeptide Y (NPY)
Arcuate nucleus
Internal medicine
medicine
Animals
GHRELIN
Biological Psychiatry
γ-aminobutyric acid (GABA)
Γ-AMINOBUTYRIC ACID (GABA)
Endocrine and Autonomic Systems
Arcuate Nucleus of Hypothalamus
030104 developmental biology
nervous system
Ciencias Médicas
Corticosterone
Neuroscience
030217 neurology & neurosurgery
Paraventricular Hypothalamic Nucleus
Subjects
Details
- ISSN :
- 03064530
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Psychoneuroendocrinology
- Accession number :
- edsair.doi.dedup.....b506e7f396b17bcc35dea1113d370ed8