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The HC-Pro Protein of Potato Virus Y Interacts with NtMinD of Tobacco
- Source :
- Molecular Plant-Microbe Interactions®. 20:1505-1511
- Publication Year :
- 2007
- Publisher :
- Scientific Societies, 2007.
-
Abstract
- Potato virus Y (PVY) infections often lead to altered numbers of host plant chloroplasts, as well as changes in morphology and inhibited photosynthesis. The multifunctional protein helper component-proteinase, HC-Pro, has been identified in PVY-infected leaf chloroplasts. We used yeast two-hybrid and bimolecular fluorescence complementation assays to demonstrate that HC-Pro can interact with the chloroplast division-related factor NtMinD in yeast and tobacco cells, respectively. In addition, we confirmed that residues 271 to 314 in NtMinD are necessary for its interaction with PVY HC-Pro in a yeast two-hybrid analysis using four NtMinD deletion mutants. These residues are necessary for the dimerization of NtMinD, which plays a vital role in chloroplast division. Thus, PVY HC-Pro may affect NtMinD activity by inhibiting the formation of NtMinD homodimers, and this may interfere with chloroplast division and contribute to changes in the numbers of chloroplast per cell observed in PVY-infected plants.
- Subjects :
- Chloroplasts
Physiology
Nicotiana tabacum
Molecular Sequence Data
Potyvirus
Biology
Viral Proteins
Bimolecular fluorescence complementation
Two-Hybrid System Techniques
Protein Interaction Mapping
Tobacco
Amino Acid Sequence
Plastid
Gene Library
Plant Proteins
food and beverages
General Medicine
biology.organism_classification
Virology
Yeast
Chloroplast
Complementation
Cysteine Endopeptidases
Biochemistry
Potato virus Y
Dimerization
Sequence Alignment
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 19437706 and 08940282
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Molecular Plant-Microbe Interactions®
- Accession number :
- edsair.doi.dedup.....b505866ef9d2a6a603269bb96187faf7