Back to Search
Start Over
Remedy of contamination of multidrug resistant Salmonella and Escherichia coli from betel leaves (Piper betle) keeping them fresh for long time
- Source :
- Journal of Advanced Veterinary and Animal Research, Vol 5, Iss 1, Pp 73-80 (2018)
- Publication Year :
- 2018
- Publisher :
- Network for the Veterinarians of Bangladesh, 2018.
-
Abstract
- Objective: The present study was carried out to identify the associated Salmonella and Escherichia coli in betel leaves ( Piper betle ), and to develop an effective method to remove those microbes. Materials and methods: Betel leaves were collected from local and whole sale markets, and borouj (cultivation place). Salmonella and E. coli were isolated and identified by cultural, morphological, and biochemical tests followed by confirmation by polymerase chain reaction (PCR) targeting the genus specific 16S rRNA genes. Antibiogram of the isolated bacteria was performed by disc diffusion method. Different concentrations of Salmosan-A Soln were used to remediate the contaminating bacteria keeping the quality of betel leaves for longer periods. Results: Total Salmonella counts in the betel leaves were 3.9×10 5 , 4.9×10 6 , 3.5×10 4 , 1.1×10 3 and 1.5×10 3 CFU/mL, while E. coli counts were 5.5×10 7 , 6.3×10 7 , 4.4×10 5 , 3.3×10 3 and 3.1×10 3 CFU/mL in the betel leaves collected from K.R. market, Kewatkhali Bazaar, whole sale market, borouj in Kushtia and borouj in Natore, respectively. Antibiogram study revealed that the isolated bacteria were sensitive to doxycyclline, ciprofloxacin, chloramphenicol and cefotaxime. Application of 0.3% Salmosan-A Soln was found to be the most effective and suitable, where
- Subjects :
- 0301 basic medicine
Veterinary medicine
Salmonella
Piper
Cefotaxime
lcsh:Veterinary medicine
General Veterinary
biology
Chloramphenicol
030106 microbiology
medicine.disease_cause
Betel
biology.organism_classification
Betel leaves
Contamination
Escherichia coli
Treatment
Ciprofloxacin
03 medical and health sciences
medicine
lcsh:SF600-1100
Animal Science and Zoology
Bacteria
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 23117710
- Volume :
- 5
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Advanced Veterinary and Animal Research
- Accession number :
- edsair.doi.dedup.....b4ed55bb19d30261820fcf2bdf757698