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Remedy of contamination of multidrug resistant Salmonella and Escherichia coli from betel leaves (Piper betle) keeping them fresh for long time

Authors :
Mohammad Ferdousur Rahman Khan
Mostakin Ahamed
Md. Bahanur Rahman
Md. Liakot Hossen
K. H. M. Nazmul Hussain Nazir
Md. Shafiullah Parvej
Tazrin Kamal
Wahedul Karim Ansari
Marzia Rahman
Md. Tanvir Rahman
Sayedun Nahar Panna
Sultan Ahmed
Source :
Journal of Advanced Veterinary and Animal Research, Vol 5, Iss 1, Pp 73-80 (2018)
Publication Year :
2018
Publisher :
Network for the Veterinarians of Bangladesh, 2018.

Abstract

Objective: The present study was carried out to identify the associated Salmonella and Escherichia coli in betel leaves ( Piper betle ), and to develop an effective method to remove those microbes. Materials and methods: Betel leaves were collected from local and whole sale markets, and borouj (cultivation place). Salmonella and E. coli were isolated and identified by cultural, morphological, and biochemical tests followed by confirmation by polymerase chain reaction (PCR) targeting the genus specific 16S rRNA genes. Antibiogram of the isolated bacteria was performed by disc diffusion method. Different concentrations of Salmosan-A Soln were used to remediate the contaminating bacteria keeping the quality of betel leaves for longer periods. Results: Total Salmonella counts in the betel leaves were 3.9×10 5 , 4.9×10 6 , 3.5×10 4 , 1.1×10 3 and 1.5×10 3 CFU/mL, while E. coli counts were 5.5×10 7 , 6.3×10 7 , 4.4×10 5 , 3.3×10 3 and 3.1×10 3 CFU/mL in the betel leaves collected from K.R. market, Kewatkhali Bazaar, whole sale market, borouj in Kushtia and borouj in Natore, respectively. Antibiogram study revealed that the isolated bacteria were sensitive to doxycyclline, ciprofloxacin, chloramphenicol and cefotaxime. Application of 0.3% Salmosan-A Soln was found to be the most effective and suitable, where

Details

Language :
English
ISSN :
23117710
Volume :
5
Issue :
1
Database :
OpenAIRE
Journal :
Journal of Advanced Veterinary and Animal Research
Accession number :
edsair.doi.dedup.....b4ed55bb19d30261820fcf2bdf757698