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Epigenetic modifications and chromatin loop organization explain the different expression profiles of the Tbrg4, WAP and Ramp3 genes
- Source :
- Experimental Cell Research, Experimental Cell Research, Elsevier, 2008, 314 (5), pp.975-987. ⟨10.1016/j.yexcr.2008.01.001⟩
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- International audience; Whey Acidic Protein (WAP) gene expression is specific to the mammary gland and regulated by lactogenic hormones to peak during lactation. It differs markedly from the more constitutive expression of the two flanking genes, Ramp3 and Tbrg4. Our results show that the tight regulation of WAP gene expression parallels variations in the chromatin structure and DNA methylation profile throughout the Ramp3–WAP–Tbrg4 locus. Three Matrix Attachment Regions (MAR) have been predicted in this locus. Two of them are located between regions exhibiting open and closed chromatin structures in the liver. The third, located around the transcription start site of the Tbrg4 gene, interacts with topoisomerase II in HC11 mousemammary cells, and in these cells anchors the chromatin loop to the nuclear matrix. Furthermore, if lactogenic hormones are present in these cells, the chromatin loop surrounding theWAPgene is more tightly attached to the nuclear structure, as observed after a high salt treatment of the nuclei and the formation of nuclear halos. Taken together, our results point to a combination of several epigenetic events that may explain the differential expression pattern of the WAP locus in relation to tissue and developmental stages
- Subjects :
- LACTATION
[SDV]Life Sciences [q-bio]
PROLACTIN
Receptor Activity-Modifying Protein 3
Receptor Activity-Modifying Proteins
Cell Line
03 medical and health sciences
Mice
0302 clinical medicine
Mammary Glands, Animal
MILK
Animals
[INFO]Computer Science [cs]
Scaffold/matrix attachment region
ChIA-PET
030304 developmental biology
Regulation of gene expression
0303 health sciences
biology
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Cell Biology
DNA Methylation
Nuclear matrix
Milk Proteins
Molecular biology
Chromatin
DIFFERENTIATION
Gene Expression Regulation
Liver
030220 oncology & carcinogenesis
DNA methylation
biology.protein
Chromatin Loop
Whey Acidic Protein
Rabbits
CHROMATIN STRUCTURE
Subjects
Details
- Language :
- English
- ISSN :
- 00144827 and 10902422
- Database :
- OpenAIRE
- Journal :
- Experimental Cell Research, Experimental Cell Research, Elsevier, 2008, 314 (5), pp.975-987. ⟨10.1016/j.yexcr.2008.01.001⟩
- Accession number :
- edsair.doi.dedup.....b4ccdb26fcd7382c2cc035e664eae2d1