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Influence of Spray-dried Hydroxyapatite-5-Fluorouracil Granules on Cell Lines Derived from Tissues of Mesenchymal Origin
- Source :
- Molecules, Molecules, Vol 13, Iss 11, Pp 2729-2739 (2008), Molecules; Volume 13; Issue 11; Pages: 2729-2739
- Publication Year :
- 2008
- Publisher :
- MDPI, 2008.
-
Abstract
- In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.
- Subjects :
- Cell type
Antimetabolites, Antineoplastic
5-Fluorouracil
Drug delivery system
Pharmaceutical Science
in Vitro cytotoxicity
Article
Analytical Chemistry
Hydroxyapatite
Cell Line
lcsh:QD241-441
Mesoderm
Mice
lcsh:Organic chemistry
Spray dried particles
Cell Line, Tumor
Drug Discovery
medicine
Animals
Cell Lineage
Physical and Theoretical Chemistry
Cell Proliferation
Osteosarcoma
Dose-Response Relationship, Drug
Cell growth
Chemistry
Organic Chemistry
Mesenchymal stem cell
Fibroblasts
medicine.disease
In vitro
Microspheres
Rats
Durapatite
Chemistry (miscellaneous)
Cell culture
Spray drying
Immunology
Drug delivery
Biophysics
Microscopy, Electron, Scanning
Molecular Medicine
Fluorouracil
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 13
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....b4c9402397d3729e05d59278cbd98bb4