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Inactivated whole virus particle vaccine with potent immunogenicity and limited IL-6 induction is ideal for influenza
- Source :
- Vaccine. 37:2158-2166
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- In contrast to current ether- or detergent-disrupted "split" vaccines (SVs) for influenza, inactivated whole influenza virus particle vaccines (WPVs) retain the original virus structure and components and as such may confer similar immunity to natural infection. In a collaboration between academia and industry, the potential of WPV as a new seasonal influenza vaccine was investigated. Each of the four seasonal influenza vaccine manufacturers in Japan prepared WPVs and SVs from the same batches of purified influenza virus. Both mice and monkeys vaccinated with the WPVs exhibited superior immune responses to those vaccinated with the corresponding SVs. Vaccination with A/California/07/2009 (H1N1) WPV enabled mice to survive a lethal challenge dose of homologous virus whereas those vaccinated with SV succumbed to infection within 6 days. Furthermore, mice vaccinated with WPV induced substantial numbers of multifunctional CD8+ T cells, important for control of antigenically drifted influenza virus strains. In addition, cytokines and chemokines were detected at early time points in the sera of mice vaccinated with WPV but not in those animals vaccinated with SV. These results indicate that WPVs induce enhanced innate and adaptive immune responses compared to equivalent doses of SVs. Notably, WPV at one fifth of the dose of SV was able to induce potent immunity with limited production of IL-6, one of the pyrogenic cytokines. We thus propose that WPVs with balanced immunogenicity and safety may set a new global standard for seasonal influenza vaccines.
- Subjects :
- Male
030231 tropical medicine
Hemagglutinin (influenza)
CD8-Positive T-Lymphocytes
Antibodies, Viral
medicine.disease_cause
Virus
Mice
03 medical and health sciences
Immunogenicity, Vaccine
Influenza A Virus, H1N1 Subtype
0302 clinical medicine
Immune system
Japan
Orthomyxoviridae Infections
Immunity
Influenza, Human
Influenza A virus
medicine
Animals
Humans
030212 general & internal medicine
General Veterinary
General Immunology and Microbiology
biology
Interleukin-6
Immunogenicity
Virion
Public Health, Environmental and Occupational Health
Virology
Mice, Inbred C57BL
Vaccination
Infectious Diseases
Vaccines, Inactivated
Influenza Vaccines
biology.protein
Cytokines
Macaca
Molecular Medicine
Female
Chemokines
Antibody
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....b4c81049c83b595e3093f645381468e6