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Modification of thermosensitivity and chemosensitivity induced by combined treatments with hyperthermia and adriamycin
- Source :
- International Journal of Molecular Medicine.
- Publication Year :
- 2001
- Publisher :
- Spandidos Publications, 2001.
-
Abstract
- Both adriamycin (ADM) and hyperthermia show thermal chemo-enhancement. Tolerance induction against ADM in heated cells has been reported resulting in clinical difficulty of cancer therapy. We investigated thermo-enhancement induced with ADM (0.2 microg/ml) treatment alone or combined with ADM and 42 degrees C hyperthermia in Chinese hamster V79 cells in vitro. Intracellular accumulation of hsc70 and hsp72 proteins after hyperthermia or ADM was observed to examine the possible relationship between cell killing effect and their accumulations. Thermosensitivity of V79 cells at 42 degrees C after the simultaneous treatments with ADM showed marked thermo-enhancement within the short-term treatments for less than 1 h, while the combined treatments for longer than 1 h, the cells showed reduced thermosensitivity. Survival from the simultaneous treatments for less than 1 h was reduced markedly less than the single treatment both with ADM or 42 degrees C hyperthermia alone. Thermotolerance was markedly induced in a step-up hyperthermia (42 degrees C 2 h-44 degrees C). The combined treatments with ADM and 44 degrees C hyperthermia following the 42 degrees C preheating alone does not inhibit thermotolerance development. The combined treatments with ADM and 42 degrees C preheating showed markedly interactive cell killing, but no thermo-enhancement to the following 44 degrees C hyperthermia was shown. The leveling slope of the 44 degrees C heating period-survival curve was drawn. In the Western blot analyses, hsc70 existed constitutively in the V79 cells. Following the 42 or 44 degrees C hyperthermia alone, intracellular accumulation of hsp72 was determined. ADM treatment alone did not induce any accumulation of hsp72. In the simultaneous treatments with ADM and hyperthermia, the accumulation of hsp72 was markedly reduced. The accumulation of hsp72 after the combined treatment with ADM and hyperthermia was not observed as markedly as that after hyperthermia alone.
- Subjects :
- Hyperthermia
Hot Temperature
Time Factors
Cell Survival
Antineoplastic Agents
HSP72 Heat-Shock Proteins
Pharmacology
Chinese hamster
Cell Line
Genetics
Animals
Medicine
Heat-Shock Proteins
biology
business.industry
General Medicine
biology.organism_classification
medicine.disease
Adaptation, Physiological
Molecular biology
In vitro
Tolerance induction
Cell killing
Doxorubicin
Cell culture
Apoptosis
business
Intracellular
Subjects
Details
- ISSN :
- 1791244X and 11073756
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Medicine
- Accession number :
- edsair.doi.dedup.....b4c75a9e3b90a56802fb9ea48593fc0c