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Permeability of dihydro- and cysteine-brevetoxin metabolites across a Caco-2 cell monolayer
- Source :
- Harmful Algae, Harmful Algae, Elsevier, 2014, 32, pp.22-26. ⟨10.1016/j.hal.2013.11.007⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Brevetoxin B is a highly reactive molecule, due in part to an α,β-unsaturated aldehyde group at the terminal side chain, leading to metabolism by reduction, oxidation and conjugation. These reactions have little effect to reduce intrinsic activity at the voltage-gated sodium channel or during toxicity testing by either enzyme-linked immunosorbent assay or mouse bioassay. Here we investigate the potential for intestinal absorption of the two most abundant brevetoxins present in Gulf of Mexico oysters using human Caco-2 cell monolayers, a widely utilized in vitro test to predict oral bioavailability of drugs and their metabolites. We found that both dihydrobrevetoxin B and cysteine brevetoxin B were rapidly taken up by the Caco-2 monolayer. However, only dihydrobrevetoxin B passes through the monolayer to reach the basal compartment. Dihydrobrevetoxin B has a moderate apparent permeability coefficient of 1.6 × 10 −6 cm/s at 500 ng/mL and nearly 50% of the toxin passes from the apical to basal compartment in 24 h. The inability of the cysteine brevetoxin B to pass through an intestinal epithelial barrier suggests that this bioactive brevetoxin metabolite that persists in shellfish may not contribute to neurotoxic shellfish poisoning.
- Subjects :
- marine toxin
Metabolite
Neurotoxic shellfish poisoning
Plant Science
Aquatic Science
Intestinal absorption
03 medical and health sciences
Brevetoxin
chemistry.chemical_compound
toxin
030304 developmental biology
0303 health sciences
brevetoxine
030302 biochemistry & molecular biology
toxicologie
Metabolism
toxine
chemistry
Biochemistry
Caco-2
caco-2
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Marine toxin
Cysteine
brevetoxin
toxicology
Subjects
Details
- Language :
- English
- ISSN :
- 15689883
- Database :
- OpenAIRE
- Journal :
- Harmful Algae, Harmful Algae, Elsevier, 2014, 32, pp.22-26. ⟨10.1016/j.hal.2013.11.007⟩
- Accession number :
- edsair.doi.dedup.....b4a8ceedace0fc32fa949d33691f5216