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In Vitro and In Silico Evaluation for the Inhibitory Action of O. basilicum Methanol Extract on α-Glucosidase and α-Amylase
- Source :
- Evidence-Based Complementary and Alternative Medicine, Vol 2021 (2021), Evidence-based Complementary and Alternative Medicine : eCAM
- Publication Year :
- 2021
- Publisher :
- Hindawi, 2021.
-
Abstract
- Diabetes mellitus is a metabolic disease that predominates, nowadays. It causes hyperglycemia and consequently major health complications. Type II diabetes is the most common form and is a result of insulin resistance in the target tissues. To treat this disease, several mechanisms have been proposed. The most direct route is via inhibiting the intestinal enzymes, e.g., α-glucosidase and α-amylase, responsible for intestinal polysaccharide digestion that therefore would reduce the absorption of monosugars through the intestinal walls. In this study, we shed the light on this route by testing the inhibitory effect of Ocimum basilicum extract on the enzymes α-glucosidase and α-amylase in vitro and in silico. Experimental procedures were performed to test the effect of the O. basilicum methanol extract from aerial parts followed by the in silico docking. 500 μg/mL of the extract led to 70.2% ± 8.6 and 25.4% ± 3.3 inhibition on α-glucosidase and α-amylase activity, respectively. Similarly, the effect of caffeic acid, a major extract ingredient, was also tested, and it caused 42.7% ± 3.0 and 47.1% ± 4.0 inhibition for α-amylase and α-glucosidase, respectively. Docking experiments were performed to predict the phytochemicals responsible for this robust inhibitory activity in the O. basilicum extracts. Several compounds have shown variable levels of inhibition, e.g., caffeic acid, pyroglutamic acid, and uvasol. The results indicated that O. basilicum can be a potent antidiabetic drug.
- Subjects :
- food.ingredient
Article Subject
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Other systems of medicine
food
Caffeic acid
Amylase
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
Basilicum
Ocimum
biology.organism_classification
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Enzyme
Complementary and alternative medicine
chemistry
Biochemistry
Docking (molecular)
biology.protein
Pyroglutamic acid
Polysaccharide digestion
RZ201-999
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....b4a363eac9a63dba7db5a061c7d56374
- Full Text :
- https://doi.org/10.1155/2021/5515775