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Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis
- Source :
- Science in China Series C: Life Sciences. 50:676-689
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- MADS-box genes are involved in floral organ development. Here we report that an AGL6(Agamous-like 6)-like MADS-box gene, HoAGL6, was isolated from Hyacinthus orientalis L. Expression pattern analysis demonstrated that HoAGL6 transcript was detected in inflorescence buds, tepals, carpels and ovules, but not in stamina, leaves or scales. Transgenic Arabidopsis plants ectopically expressing HoAGL6 exhibited novel phenotypes of significantly reduced plant size, extremely early flowering, and losing inflorescence indeterminacy. In addition, wide homeotic conversion of sepals, petals, and leaves into carpel-like or ovary structures, and disappearance or number reduction of stamens in 35S::HoAGL6 Arabidopsis plants were also observed. RT-PCR analysis indicated that the expressions of flowering time gene SOC1 and flower meristem identity gene LFY were significantly up-regulated in 35S::HoAGL6 transgenic Arabidopsis plants, and the expression levels of floral organ identity genes AG and SEP1 in leaves were also elevated. These results indicated that HoAGL6 was involved in the regulation of flower transition and flower organ formation.
- Subjects :
- Molecular Sequence Data
Arabidopsis
Ovary (botany)
Stamen
Flowers
Hyacinthus orientalis
General Biochemistry, Genetics and Molecular Biology
Sepal
Species Specificity
Gene Expression Regulation, Plant
Botany
Amino Acid Sequence
Hyacinthus
Phylogeny
Plant Proteins
General Environmental Science
Sequence Homology, Amino Acid
biology
fungi
food and beverages
Plants, Genetically Modified
biology.organism_classification
ABC model of flower development
Blotting, Southern
Phenotype
Petal
General Agricultural and Biological Sciences
Homeotic gene
Plasmids
Subjects
Details
- ISSN :
- 18622798 and 10069305
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Science in China Series C: Life Sciences
- Accession number :
- edsair.doi.dedup.....b49c80eef976fbdcd24f0b757c70d974