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A Comparative Proteomic Analysis of Pinellia ternata Leaves Exposed to Heat Stress
- Source :
- International Journal of Molecular Sciences; Volume 14; Issue 10; Pages: 20614-20634, International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 14, Iss 10, Pp 20614-20634 (2013)
- Publication Year :
- 2013
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2013.
-
Abstract
- Pinellia ternata is an important traditional Chinese medicinal plant. The growth of P. ternata is sensitive to high temperatures. To gain a better understanding of heat stress responses in P. ternata, we performed a comparative proteomic analysis. P. ternata seedlings were subjected to a temperature of 38 °C and samples were collected 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress was frequently generated in rice leaves exposed to high temperature. Two-dimensional electrophoresis (2-DE) was used to analyze heat-responsive proteins. More than 600 protein spots were reproducibly detected on each gel; of these spots, 20 were up-regulated, and 7 were down-regulated. A total of 24 proteins and protein species were successfully identified by MALDI-TOF/TOF MS. These proteins and protein species were found to be primarily small heat shock proteins (58%) as well as proteins involved in RNA processing (17%), photosynthesis (13%), chlorophyll biosynthetic processes (4%), protein degradation (4%) and defense (4%). Using 2-DE Western blot analysis, we confirmed the identities of the cytosolic class II small heat shock protein (sHSPs-CII) identified by MS. The expression levels of four different proteins [cytosolic class I small heat shock protein (sHSPs-CI), sHSPs-CII, mitochondrial small heat shock protein (sHSPs-MIT), glycine-rich RNA-binding protein (GRP)] were analyzed at the transcriptional level by quantitative real-time PCR. The mRNA levels of three sHSPs correlated with the corresponding protein levels. However, GRP was down-regulated at the beginning of heat stress but then increased substantially to reach a peak after 24 h of heat stress. Our study provides valuable new insight into the responses of P. ternata to heat stress.
- Subjects :
- Chlorophyll
Proteomics
Hot Temperature
Pinellia ternata
Proteome
Pinellia
Down-Regulation
Gene Expression
Protein degradation
proteomics
quantitative real-time PCR
heat stress
Catalysis
Article
lcsh:Chemistry
Inorganic Chemistry
Western blot
Stress, Physiological
Heat shock protein
Gene expression
medicine
Physical and Theoretical Chemistry
Photosynthesis
RNA Processing, Post-Transcriptional
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Heat-Shock Proteins
biology
medicine.diagnostic_test
Organic Chemistry
General Medicine
biology.organism_classification
Molecular biology
Computer Science Applications
Up-Regulation
Plant Leaves
Oxidative Stress
lcsh:Biology (General)
lcsh:QD1-999
Biochemistry
Proteolysis
Lipid Peroxidation
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 14; Issue 10; Pages: 20614-20634
- Accession number :
- edsair.doi.dedup.....b4569c346d38d1622be380fd547183a8
- Full Text :
- https://doi.org/10.3390/ijms141020614