Supplemental Experimental Procedures, Supplementary Tables 1-3, Supplementary Figures 1-9 from Global Metabolic Profiling Identifies a Pivotal Role of Proline and Hydroxyproline Metabolism in Supporting Hypoxic Response in Hepatocellular Carcinoma

Table S1. Clinical information of enrolled subjects for discovery; Table S2. Clinical information of enrolled hepatic carcinoma subjects for validation; TableS3. Differential metabolites obtained from tissue specimens in metabolomics discovery; Figure S1. Scheme of the metabolic characterization study of hepatic carcinoma; Figure S2. Metabolic pathway analysis for HCC. Figure S3. Refining of important differential metabolites; Figure S4. The correlation between hydroxyproline and clinical parameters for discovery specimens; Figure S5. Proliferation assay in tumor xenografts; Figure S6. mRNA levels of PRODH2 and ALDH18A1 in HCC under hypoxia; Figure S7. 13C5 glutamine tracer analysis for glutamate and proline production under hypoxic condition in SMMC-7721 cell line. (mean± SE); Figure S8. Knockdown of MYC has no effect on expression of prolidase in HCC cell; Figure S9. Knockdown of ALDH18A1 induced apoptosis in the presence of sorafenib under hypoxia.