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Parameter optimization toward optimal microneedle-based dermal vaccination

Authors :
Koen van der Maaden
Wim Jiskoot
Huixin Yu
Stefan Romeijn
Eleni Maria Varypataki
Joke A. Bouwstra
Source :
European Journal of Pharmaceutical Sciences. 64:18-25
Publication Year :
2014
Publisher :
Elsevier BV, 2014.

Abstract

Microneedle-based vaccination has several advantages over vaccination by using conventional hypodermic needles. Microneedles are used to deliver a drug into the skin in a minimally-invasive and potentially pain free manner. Besides, the skin is a potent immune organ that is highly suitable for vaccination. However, there are several factors that influence the penetration ability of the skin by microneedles and the immune responses upon microneedle-based immunization. In this study we assessed several different microneedle arrays for their ability to penetrate ex vivo human skin by using trypan blue and (fluorescently or radioactively labeled) ovalbumin. Next, these different microneedles and several factors, including the dose of ovalbumin, the effect of using an impact-insertion applicator, skin location of microneedle application, and the area of microneedle application, were tested in vivo in mice. The penetration ability and the dose of ovalbumin that is delivered into the skin were shown to be dependent on the use of an applicator and on the microneedle geometry and size of the array. Besides microneedle penetration, the above described factors influenced the immune responses upon microneedle-based vaccination in vivo. It was shown that the ovalbumin-specific antibody responses upon microneedle-based vaccination could be increased up to 12-fold when an impact-insertion applicator was used, up to 8-fold when microneedles were applied over a larger surface area, and up to 36-fold dependent on the location of microneedle application. Therefore, these influencing factors should be considered to optimize microneedle-based dermal immunization technologies.

Details

ISSN :
09280987
Volume :
64
Database :
OpenAIRE
Journal :
European Journal of Pharmaceutical Sciences
Accession number :
edsair.doi.dedup.....b4419323bcac149a5fc7750f0cd4a200
Full Text :
https://doi.org/10.1016/j.ejps.2014.08.004