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Parameter optimization toward optimal microneedle-based dermal vaccination
- Source :
- European Journal of Pharmaceutical Sciences. 64:18-25
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Microneedle-based vaccination has several advantages over vaccination by using conventional hypodermic needles. Microneedles are used to deliver a drug into the skin in a minimally-invasive and potentially pain free manner. Besides, the skin is a potent immune organ that is highly suitable for vaccination. However, there are several factors that influence the penetration ability of the skin by microneedles and the immune responses upon microneedle-based immunization. In this study we assessed several different microneedle arrays for their ability to penetrate ex vivo human skin by using trypan blue and (fluorescently or radioactively labeled) ovalbumin. Next, these different microneedles and several factors, including the dose of ovalbumin, the effect of using an impact-insertion applicator, skin location of microneedle application, and the area of microneedle application, were tested in vivo in mice. The penetration ability and the dose of ovalbumin that is delivered into the skin were shown to be dependent on the use of an applicator and on the microneedle geometry and size of the array. Besides microneedle penetration, the above described factors influenced the immune responses upon microneedle-based vaccination in vivo. It was shown that the ovalbumin-specific antibody responses upon microneedle-based vaccination could be increased up to 12-fold when an impact-insertion applicator was used, up to 8-fold when microneedles were applied over a larger surface area, and up to 36-fold dependent on the location of microneedle application. Therefore, these influencing factors should be considered to optimize microneedle-based dermal immunization technologies.
- Subjects :
- Microinjections
Ovalbumin
Pharmaceutical Science
Human skin
Administration, Cutaneous
Antigen-Antibody Reactions
Mice
In vivo
Animals
Humans
Medicine
Antigens
Mice, Inbred BALB C
biology
business.industry
Vaccination
Penetration (firestop)
Pain free
Antibody response
Needles
Immunoglobulin G
Immunology
biology.protein
Female
business
Ex vivo
Biomedical engineering
Subjects
Details
- ISSN :
- 09280987
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmaceutical Sciences
- Accession number :
- edsair.doi.dedup.....b4419323bcac149a5fc7750f0cd4a200
- Full Text :
- https://doi.org/10.1016/j.ejps.2014.08.004