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A gold nanoparticle-based immunochromatographic assay for simultaneous detection of multiplex sildenafil adulterants in health food by only one antibody
- Source :
- Analytica Chimica Acta. 1141:1-12
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Abstarct In the last few years, sildenafil and its analogues have emerged as an illegal ingredient in health food and products, causing illness and death. To address this problem, herein, a gold nanoparticle-based immunochromatographic assay for simultaneous detection sildenafil and its analogues was developed by using just one monoclonal antibody. This method can realize qualitative and quantitative analysis of sildenafil and its analogues with a detection range (IC20-IC80) of 0.12–28.05 ng mL−1, the IC50 was 0.31–8.45 ng mL−1 and the cut-off value was 5.0–80.0 ng mL−1. Meanwhile, this method could also accomplish semi-quantitative analysis of vardenafil with a cut-off value of 0.625 ng mL−1. The detection can be finished in 6 min and showed no cross-reactivity with avanafil and tadalafil. The recoveries of spiked samples ranged from 70.5 to 118.9% with a coefficient variation less than 15%, which confirmed the assay was sensitive, accurate and adapted to the rapid determination of sildenafil and its analogues.
- Subjects :
- Sildenafil
Metal Nanoparticles
Food Contamination
02 engineering and technology
Avanafil
01 natural sciences
Biochemistry
Chromatography, Affinity
Sildenafil Citrate
Analytical Chemistry
chemistry.chemical_compound
Limit of Detection
medicine
Environmental Chemistry
Multiplex
Health food
Spectroscopy
Immunoassay
Chromatography
biology
Chemistry
010401 analytical chemistry
021001 nanoscience & nanotechnology
Tadalafil
0104 chemical sciences
Vardenafil
biology.protein
Gold
Antibody
0210 nano-technology
Quantitative analysis (chemistry)
medicine.drug
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 1141
- Database :
- OpenAIRE
- Journal :
- Analytica Chimica Acta
- Accession number :
- edsair.doi.dedup.....b43cec2fe72b19786ac14c4ad7ec79f9