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Comparison of alternative mesenchymal stem cell sources for cell banking and musculoskeletal advanced therapies
- Source :
- Journal of cellular biochemistry, 112(5), 1418-1430. Wiley-Liss Inc.
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- With the continuous discovery of new alternative sources containing mesenchymal stem cells (MSCs), regenerative medicine therapies may find tailored applications in the clinics. Although these cells have been demonstrated to express specific mesenchymal markers and are able to differentiate into mesenchymal lineages in ad hoc culture conditions, it is still critical to determine the yield and differentiation potential of these cells in comparative studies under the same standardized culture environment. Moreover, the opportunity to use MSCs from bone marrow (BM) of multiorgan donors for cell banking is of relevant importance. In the attempt to establish the relative potential of alternative MSCs sources, we analyzed and compared the yield and differentiation potential of human MSCs from adipose and BM tissues of cadaveric origins, and from fetal annexes (placenta and umbilical cord) after delivery using standardized isolation and culture protocols. BM contained a significantly higher amount of mononuclear cells (MNCs) compared to the other tissue sources. Nonetheless, a higher cell seeding density was needed for these cells to successfully isolate MSCs. The MNCs populations were highly heterogeneous and expressed variable MSCs markers with a large variation from donor to donor. After MSCs selection through tissue culture plastic adhesion, cells displayed a comparable proliferation capacity with distinct colony morphologies and were positive for a pool of typical MSCs markers. In vitro differentiation assays showed a higher osteogenic differentiation capacity of adipose tissue and BM MSCs, and a higher chondrogenic differentiation capacity of BM MSCs.
- Subjects :
- METIS-283327
Male
Cellular differentiation
Clinical uses of mesenchymal stem cells
Adipose tissue
Bone Marrow Cells
Biology
Regenerative Medicine
bone
Biochemistry
Regenerative medicine
Tissue culture
CARTILAGE
Pregnancy
IR-104458
Cadaver
medicine
Humans
alternative source
PLACENTA
Musculoskeletal Diseases
Molecular Biology
Biological Specimen Banks
Stem cell transplantation for articular cartilage repair
allogenic therapie
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
medicine.anatomical_structure
Adipose Tissue
Immunology
Cancer research
Female
Bone marrow
Subjects
Details
- ISSN :
- 07302312
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....b437c4d9c50ed7fe1c87936b62e69521