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Targeting of folate-conjugated liposomes with co-entrapped drugs to prostate cancer cells via prostate-specific membrane antigen (PSMA)
- Source :
- Nanomedicine : nanotechnology, biology, and medicine. 14(4)
- Publication Year :
- 2017
-
Abstract
- Folate-targeted liposomes (FTL) were tested as drug delivery vehicles to PSMA-positive cancer cells. We used FL with co-entrapped mitomycin C lipophilic prodrug (MLP) and doxorubicin (DOX), and the LNCaP prostate cancer cell line which expresses PSMA but is negative for folate receptor. A major increase in cell drug levels was observed when LNCaP cells were incubated with FTL as compared to non-targeted liposomes (NTL). MLP was activated to mitomycin C, and intracellular and nuclear fluorescence of DOX was detected, indicating FTL processing and drug bioavailability. PMPA (2-(phosphonomethyl)-pentanedioic acid), a specific inhibitor of PSMA, blocked the uptake of FTL into LNCaP cells, but did not affect the uptake of FTL into PSMA-deficient and folate receptor-positive KB cells. The cytotoxic activity of drug-loaded FTL was found significantly enhanced when compared to NTL in LNCaP cells. FTL may provide a new tool for targeted therapy of cancers that over-express the PSMA receptor.
- Subjects :
- 0301 basic medicine
Glutamate Carboxypeptidase II
Male
Cell Survival
Mitomycin
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Bioengineering
urologic and male genital diseases
03 medical and health sciences
0302 clinical medicine
Drug Delivery Systems
Folic Acid
Cell Line, Tumor
LNCaP
Cytotoxic T cell
Humans
General Materials Science
Liposome
Chemistry
Mitomycin C
Prostatic Neoplasms
Prodrug
030104 developmental biology
Folate receptor
Doxorubicin
030220 oncology & carcinogenesis
Drug delivery
Cancer cell
Antigens, Surface
Liposomes
Cancer research
Molecular Medicine
Subjects
Details
- ISSN :
- 15499642
- Volume :
- 14
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Accession number :
- edsair.doi.dedup.....b41e3e3a66c0d08476fbf62db99a9bbf