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Cigarette smoke total particulate matter increases mucous secreting cell numbers in vitro: A potential model of goblet cell hyperplasia
- Source :
- Toxicology in Vitro. 24:981-987
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Cigarette smoking is associated with chronic obstructive pulmonary disease (COPD)--a term encompassing chronic lung inflammation, chronic bronchitis and emphysema. Goblet cell hyperplasia is a characteristic feature of the lung epithelium in COPD patients contributing to the overproduction of airway mucus, including mucin MUC5AC. Using an in vitro model of differentiated lung epithelium we have investigated morphological and cellular changes in response to interleukin (IL)-13 (2.5-20 ng/ml), cigarette smoke total particulate matter (TPM; 0.31-20 microg/ml) and three mainstream cigarette smoke constituents: acrolein, formaldehyde and acetaldehyde (all at 0.001-1 microM) over 28 days during differentiation (agents replaced daily Monday-Friday). Control cultures of human bronchial epithelial cells (HBECs) underwent mucociliary differentiation producing a columnar epithelium containing goblet, ciliated and basal cells. Non-cytotoxic doses of IL-13 induced a significant increase in the percentage of MUC5AC positive cells. Using both flow cytometry and immunocytochemical techniques for identification of MUC5AC positive cells, TPM treatment induced an increase in MUC5AC positive cells, becoming maximal at 5 microg/ml. Acrolein treatment also increased the percentage of MUC5AC positive cells. However, formaldehyde or acetaldehyde had little effect. This study demonstrates that lung toxicants can induce a profound effect on cellular differentiation in an in vitro model of the human lung epithelium.
- Subjects :
- Adult
Chronic bronchitis
Pathology
medicine.medical_specialty
Cell Survival
Cellular differentiation
Cell Count
Acetaldehyde
Mucin 5AC
Biology
Toxicology
Formaldehyde
Smoke
Tobacco
Electric Impedance
medicine
Humans
Acrolein
Coloring Agents
Cells, Cultured
Cell Size
COPD
Interleukin-13
Lung
Mucin
Interleukin
Cell Differentiation
General Medicine
respiratory system
Flow Cytometry
medicine.disease
Immunohistochemistry
Mucus
Epithelium
Microscopy, Electron
medicine.anatomical_structure
Neutral Red
Particulate Matter
Goblet Cells
Subjects
Details
- ISSN :
- 08872333
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Toxicology in Vitro
- Accession number :
- edsair.doi.dedup.....b40849a3a8bea5c19db2c8fa00b94f04