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AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
- Source :
- Nature Communications, Nature Communications, Vol 8, Iss 1, Pp 1-17 (2017)
- Publication Year :
- 2015
-
Abstract
- A defining hallmark of primary and metastatic cancers is the migration and invasion of malignant cells. These invasive properties involve altered dynamics of the cytoskeleton and one of its major structural components β-actin. Here we identify AIM1 (absent in melanoma 1) as an actin-binding protein that suppresses pro-invasive properties in benign prostate epithelium. Depletion of AIM1 in prostate epithelial cells increases cytoskeletal remodeling, intracellular traction forces, cell migration and invasion, and anchorage-independent growth. In addition, decreased AIM1 expression results in increased metastatic dissemination in vivo. AIM1 strongly associates with the actin cytoskeleton in prostate epithelial cells in normal tissues, but not in prostate cancers. In addition to a mislocalization of AIM1 from the actin cytoskeleton in invasive cancers, advanced prostate cancers often harbor AIM1 deletion and reduced expression. These findings implicate AIM1 as a key suppressor of invasive phenotypes that becomes dysregulated in primary and metastatic prostate cancer.<br />Invasion of malignant cells involves changes in cytoskeleton dynamics. Here the authors identify absent in melanoma 1 as an actin binding protein and show that it regulates cytoskeletal remodeling and cell migration in prostate epithelial cells, acting as a metastatic suppressor in cancer cells.
- Subjects :
- 0301 basic medicine
Male
Science
Transplantation, Heterologous
General Physics and Astronomy
General Biochemistry, Genetics and Molecular Biology
Article
Cell Line
03 medical and health sciences
Prostate cancer
Mice
Microscopy, Electron, Transmission
Prostate
Cell Movement
Cell Line, Tumor
medicine
Animals
Humans
Neoplasm Invasiveness
Actin-binding protein
Cytoskeleton
Multidisciplinary
biology
HEK 293 cells
Membrane Proteins
Prostatic Neoplasms
Cell migration
General Chemistry
medicine.disease
Actin cytoskeleton
Crystallins
Actins
3. Good health
Cell biology
Transplantation
Actin Cytoskeleton
030104 developmental biology
medicine.anatomical_structure
HEK293 Cells
Microscopy, Fluorescence
Neoplasm Micrometastasis
biology.protein
RNA Interference
Protein Binding
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature communications
- Accession number :
- edsair.doi.dedup.....b3fe11c0fc3d0fd477a6b99f44dfb430