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Feasibility of Using Early Mesencephalic Neural Plate for Intracerebral Grafting
- Source :
- Cell Transplantation, Vol 11 (2002)
- Publication Year :
- 2002
- Publisher :
- SAGE Publications, 2002.
-
Abstract
- The purpose of this study was to elucidate the biological significance and the possibility of intracerebral grafting of neuroepithelial stem cells derived from the mesencephalic neural plate. Immunohistological studies of embryonic day 10.5 (E10.5) Wister rats revealed strong nestin expression in the mesencephalic part of the neural plate. Mesencephalic neural plates removed from E10.5 rats were processed to either tissue or cell dissociation culture. They were cultured in vitro under various conditions and were analyzed 7 days after the primary culture. When they were cultured as a tissue, cell proliferation and differentiation into neurons extending long neurites were obvious in a serum-free medium, in a medium containing 3% serum, and in a medium containing 20 ng/ml epidermal growth factor. On the other hand, in a medium containing 10 ng/ml basic fibroblast growth factor (bFGF), both vigorous cell proliferation and sphere formation were recognized. Furthermore, marked neurite growth was rarely seen in this culture. When they were plated in a dissociation culture, cell proliferation and neurosphere generation were also recognized only in a medium containing bFGF, depending on the initial cell concentration. The spheres, generated 7 days after the primary cell culture, were positively stained by nestin. These data suggested that bFGF was able to amplify the stem cell population present in the mesencephalic neural plate derived from early embryos. This might make it possible to obtain a large number of stem cells as donor material for neural transplantation on demand.
- Subjects :
- Telencephalon
0301 basic medicine
Neurite
Basic fibroblast growth factor
Biomedical Engineering
lcsh:Medicine
Nerve Tissue Proteins
Culture Media, Serum-Free
Nestin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Intermediate Filament Proteins
Fetal Tissue Transplantation
Mesencephalon
Culture Techniques
Neurosphere
Animals
Brain Tissue Transplantation
Rats, Wistar
Cells, Cultured
Transplantation
Epidermal Growth Factor
lcsh:R
Cell Differentiation
Cell Biology
Immunohistochemistry
Rats
Cell biology
Neuroepithelial cell
Microscopy, Electron
030104 developmental biology
chemistry
Cell culture
Feasibility Studies
Female
Stem cell
Neural plate
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15553892 and 09636897
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Cell Transplantation
- Accession number :
- edsair.doi.dedup.....b3e4cf0dcf0ba1c3eb911c8d1c7ca206