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Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization
- Source :
- International Journal of Biological Macromolecules. 167:85-92
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructose-rich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7 ± 0.1 mol% 3-hydroxydecanoate, 17.9 ± 1.0 mol% 3-hydroxyoctanoate, 14.5 ± 1.1 mol% 3-hydroxybutyrate, 11.1 ± 0.6 mol% 3-hydroxytetradecanoate, 10.1 ± 0.5 mol% 3-hydroxydodecanoate and 3.7 ± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0 ± 0.1 °C, respectively), and a degradation temperature of 300.0 ± 0.1 °C, coupled to a low crystallinity index (12.7 ± 2.7%), a molecular weight (Mw) of 1.34 × 105 ± 0.18 × 105 Da and a polydispersity index of 2.70 ± 0.03. The biopolymer's films were dense and had a smooth surface, as demonstrated by Scanning Electron Microscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging.
- Subjects :
- Chemical Phenomena
polyhydroxyalkanoates (PHA)
Dispersity
fruit pulp waste
02 engineering and technology
Chemical Fractionation
engineering.material
Biochemistry
Polyhydroxyalkanoates
03 medical and health sciences
Crystallinity
Biopolymers
Bioreactors
Structural Biology
Pseudomonas
Food science
Molecular Biology
Mechanical Phenomena
030304 developmental biology
Waste Products
chemistry.chemical_classification
0303 health sciences
biology
Polymer characterization
Pulp (paper)
General Medicine
Polymer
Pseudomonas chlororaphis
021001 nanoscience & nanotechnology
biology.organism_classification
Molecular Weight
Kinetics
chemistry
Fruit
Malus
Fermentation
engineering
Biopolymer
0210 nano-technology
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 167
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....b3ba2ed85f17d6f4c8e37c02071af2b2