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Apoptosis progression studied using parallel dielectrophoresis electrophysiological analysis and flow cytometry
- Source :
- Integrative Biology. 7:1396-1401
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Apoptosis is characterised by many cellular events, but the standard Annexin-V assay identifies two; the transfer of the phospholipid phosphatidylserine (PS) from inner to outer leaflets of the plasma membrane, acting as an "eat me" signal to macrophages, and the permeabilisation of the plasma membrane. In this paper we compare the results from the Annexin-V assay with electrophysiology data obtained in parallel using dielectrophoresis, which highlights two changes in cell electrophysiology; a change in cytoplasmic conductivity which correlates with PS expression, and a membrane conductance spike that correlates with permeabilisation. Combining results from both methods shows a strong inverse relationship between conductivity and PS externalisation. One mechanism which may explain this correlation is related to intracellular Ca(2+), which is known to increase early in apoptosis. PS expression occurs when enzymes called scramblases swap external and internal phospholipids, and which are usually activated by Ca(2+), whilst the change in cytoplasmic conductivity may be due to K(+) efflux from intermediate conductance (IK) ion channels that are also activated by Ca(2+).
- Subjects :
- Electrophoresis
Cytoplasm
Phospholipid scramblase
Biophysics
Apoptosis
Phosphatidylserines
Biology
Biochemistry
Flow cytometry
Jurkat Cells
chemistry.chemical_compound
Annexin
medicine
Humans
Annexin A5
Phospholipids
Ion channel
medicine.diagnostic_test
Cell Membrane
Phosphatidylserine
Flow Cytometry
Staurosporine
Cell biology
Electrophysiology
chemistry
Calcium
Intracellular
Propidium
Subjects
Details
- ISSN :
- 17579708 and 17579694
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Integrative Biology
- Accession number :
- edsair.doi.dedup.....b3b9502b4b04a76397a985405727e429