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Cholera toxin B subunit promotes the induction of regulatory T cells by preventing human dendritic cell maturation
- Source :
- Journal of leukocyte biology. 84(3)
- Publication Year :
- 2008
-
Abstract
- Cholera toxin B subunit (CTB) is an efficient mucosal carrier molecule for the generation of immune responses to linked antigens. There is also good evidence that CTB acts as an immunosuppressant, as it is able to down-modulate human monocyte/macrophage cell line activation and to suppress Th1-type responses. In the present study, we examined the possibility that recombinant CTB (rCTB) may affect human dendritic cell (DC) functions in response to LPS stimulation and may induce the generation of DC with the capacity to generate CD4+ regulatory T cells (Tregs). Our findings show that rCTB partially prevents the LPS-induced maturation process of monocyte-derived DC (MDDC) and decreases their IL-12 production with no relevant effect on IL-10 production. LPS-stimulated MDDC pretreated with rCTB are able to promote the induction of low proliferating T cells, which show an enhanced IL-10 production associated with a reduced IFN-γ production and the same high levels of TGF-β as the control. These T cells suppress proliferation of activated autologous T cells. Transwell experiments and blockade of IL-10R and TGF-β showed that the immunomodulatory effect is mediated by soluble factors. Thus, T cells induced by rCTB-conditioned MDDC acquire a regulatory phenotype and activity similar to those described for type 1 Tregs.
- Subjects :
- CD4-Positive T-Lymphocytes
Lipopolysaccharides
Cholera Toxin
Immunology
Stimulation
Biology
Lymphocyte Activation
T-Lymphocytes, Regulatory
Monocytes
Poisons
law.invention
Interferon-gamma
Immune system
Antigen
law
medicine
Immunology and Allergy
Humans
Antigen-presenting cell
Cells, Cultured
Cell Proliferation
Monocyte
Cell Biology
Dendritic cell
Dendritic Cells
Th1 Cells
Flow Cytometry
Molecular biology
Interleukin-12
Recombinant Proteins
Interleukin-10
medicine.anatomical_structure
Recombinant DNA
Transforming growth factor
Subjects
Details
- ISSN :
- 07415400
- Volume :
- 84
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of leukocyte biology
- Accession number :
- edsair.doi.dedup.....b3b0ac14b0ae476881dcb70586a26c96