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Human visfatin expression: relationship to insulin sensitivity, intramyocellular lipids, and inflammation
- Source :
- The Journal of clinical endocrinology and metabolism. 92(2)
- Publication Year :
- 2006
-
Abstract
- Visfatin (VF) is a recently described adipokine preferentially secreted by visceral adipose tissue (VAT) with insulin mimetic properties.The aim of this study was to examine the association of VF with insulin sensitivity, intramyocellular lipids (IMCL), and inflammation in humans.VF mRNA was examined in paired samples of VAT and abdominal sc adipose tissue (SAT) obtained from subjects undergoing surgery. Plasma VF and VF mRNA was also examined in SAT and muscle tissue, obtained by biopsy from well-characterized subjects with normal or impaired glucose tolerance, with a wide range in body mass index (BMI) and insulin sensitivity (S(I)).The study was conducted at a University Hospital and General Clinical Research Center.S(I) was measured, and fat and muscle biopsies were performed. In impaired glucose tolerance subjects, these procedures were performed before and after treatment with pioglitazone or metformin.We measured the relationship between VF and obesity, S(I), adipose tissue inflammation, IMCL, and response to insulin sensitizers.No significant difference in VF mRNA was seen between SAT and VAT depots. VAT VF mRNA associated positively with BMI, whereas SAT VF mRNA decreased with BMI. SAT VF correlated positively with S(I), and the association of SAT VF mRNA with S(I) was independent of BMI. IMCL and markers of inflammation (adipose CD68 and plasma TNFalpha) were negatively associated with SAT VF. Impaired glucose tolerance subjects treated with pioglitazone showed no change in SAT VF mRNA despite a significant increase in S(I). Plasma VF and muscle VF mRNA did not correlate with BMI or S(I) or IMCL, and there was no change in muscle VF with either pioglitazone or metformin treatments.SAT VF is highly expressed in lean, more insulin-sensitive subjects and is attenuated in subjects with high IMCL, low S(I), and high levels of inflammatory markers. VAT VF and SAT VF are regulated oppositely with BMI.
- Subjects :
- medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Biopsy
Clinical Biochemistry
Abdominal Fat
Adipose tissue
Adipokine
Gene Expression
Context (language use)
Biochemistry
Article
Body Mass Index
Impaired glucose tolerance
Endocrinology
Insulin resistance
Internal medicine
Glucose Intolerance
medicine
Humans
Hypoglycemic Agents
Obesity
RNA, Messenger
Intramyocellular lipids
Muscle, Skeletal
Nicotinamide Phosphoribosyltransferase
Inflammation
Pioglitazone
business.industry
Biochemistry (medical)
medicine.disease
Lipid Metabolism
Metformin
Cytokines
Thiazolidinediones
Insulin Resistance
business
Biomarkers
medicine.drug
Subjects
Details
- ISSN :
- 0021972X
- Volume :
- 92
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of clinical endocrinology and metabolism
- Accession number :
- edsair.doi.dedup.....b3ac1f22f60d7edea26315d6b4493074