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Competitive adsorption of fibronectin and albumin on hydroxyapatite nanocrystals
- Source :
- Science and technology of advanced materials. 12(3)
- Publication Year :
- 2010
-
Abstract
- Competitive adsorption of two-component solutions containing fibronectin (Fn) and albumin (Ab) on hydroxyapatite (HAp) nanocrystals was analyzed in situ using the quartz crystal microbalance with dissipation (QCM-D) technique. Adsorption of the one-component protein (Fn or Ab) and the two-component proteins adjusted to different molar ratios of Fn to Ab at a fixed Fn concentration was investigated. The frequency shift (Δf; Hz) and the dissipation energy shift (ΔD) were measured with the QCM-D technique, and the viscoelastic changes of adlayers were evaluated by the saturated ΔD/Δf value and the Voigt-based viscoelastic model. For the adsorption of the one-component protein, the Fn adlayer showed a larger mass and higher viscoelasticity than the Ab adlayer, indicating the higher affinity of Fn on HAp. For the adsorption of the two-component proteins, the viscoelastic properties of the adlayers became elastic with increase in Ab concentration, whereas the adsorption mass was similar to that of Fn in the one-component solution regardless of the Ab concentration. The specific binding mass of the Ab antibody to the adlayers increased with increase in Ab concentration, whereas that of the Fn antibody decreased. Therefore, Fn preferentially adsorbs on HAp and Ab subsequently interacts with the adlayers, indicating that the interfacial viscoelasticity of the adlayers was dominated by the interaction between Fn and Ab.
- Subjects :
- In situ
Materials science
Focus Papers
Competitive adsorption
biology
0206 medical engineering
Analytical chemistry
Albumin
02 engineering and technology
Quartz crystal microbalance
021001 nanoscience & nanotechnology
020601 biomedical engineering
Viscoelasticity
Fibronectin
Adsorption
Nanocrystal
biology.protein
General Materials Science
0210 nano-technology
Subjects
Details
- ISSN :
- 14686996
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Science and technology of advanced materials
- Accession number :
- edsair.doi.dedup.....b3a69cc011a01cfa4035f8b3b98c3d54