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A new ionic liquid–water–organic solvent three phase microextraction for simultaneous preconcentration flavonoids and anthraquinones from traditional Chinese prescription
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 86:36-39
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- A novel technique, ionic liquid-water-organic solvent three phase microextraction (ILWOS-3p-ME) was developed and introduced for simultaneous preconcentration and determination of flavonoids and anthraquinones in Chinese herbal formula and its preparations. This technique was performed in one step by using a syringe. High performance liquid chromatography with an UV-detector (HPLC/UV) was subsequently conducted. Two solvents with different densities (organic solvent and ionic liquid with densities less than and higher than water, respectively) were separately placed in a syringe, which was used as an extraction device. A cloudy emulsion was formed by manually shaking the syringe. The mixture was allowed to stand for several minutes; afterward, the emulsion readily separated into three phases: an upper organic solvent extraction phase; a middle aqueous sample phase; and a lower ionic liquid extraction phase. Both the upper and lower layers were transferred to a small Eppendorf (EP) tube. Conducting ILWOS-3P-ME with HPLC/UV, we simultaneously determined the bioactive components of flavonoids and anthraquinones in traditional Chinese medicine. ILWOS-3P-ME is a simple, rapid, practical, and effective method to extract and preconcentrate of different types of trace bioactive components from traditional Chinese medicine simultaneously.
- Subjects :
- Flavonoids
Chromatography
Aqueous solution
Liquid Phase Microextraction
Chemistry
Clinical Biochemistry
Extraction (chemistry)
Ionic Liquids
Water
Pharmaceutical Science
Anthraquinones
High-performance liquid chromatography
Analytical Chemistry
Solvent
chemistry.chemical_compound
Phase (matter)
Drug Discovery
Emulsion
Ionic liquid
Solvents
Chromatography, High Pressure Liquid
Spectroscopy
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....b399806c9144ddcbaeb26eb3959d9916