Back to Search
Start Over
Heterologous biosynthesis and characterization of a glycocin from a thermophilic bacterium
- Source :
- Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019), Nature Communications, Nature communications, London : Nature Publishing Group, 2019, vol. 10, art. no 1115, p. [1-12], Nature Communications, 10(1):1115. Nature Publishing Group
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- The genome of the thermophilic bacterium, Aeribacillus pallidus 8, encodes the bacteriocin pallidocin. It belongs to the small class of glycocins and is posttranslationally modified, containing an S-linked glucose on a specific Cys residue. In this study, the pallidocin biosynthetic machinery is cloned and expressed in Escherichia coli to achieve its full biosynthesis and modification. It targets other thermophilic bacteria with potent activity, demonstrated by a low minimum inhibitory concentration (MIC) value. Moreover, the characterized biosynthetic machinery is employed to produce two other glycopeptides Hyp1 and Hyp2. Pallidocin and Hyp1 exhibit antibacterial activity against closely related thermophilic bacteria and some Bacillus sp. strains. Thus, heterologous expression of a glycocin biosynthetic gene cluster including an S-glycosyltransferase provides a good tool for production of hypothetical glycocins encoded by various bacterial genomes and allows rapid in vivo screening.<br />Heterologous production of the glycocins, posttranslationally modified peptide bacteriocins containing a sugar moiety, has not been achieved. Here, the authors express a thermophilic bacterium glycocin biosynthetic gene cluster and S-glycosyltransferase for the production of antibacterial glycocins in E. coli.
- Subjects :
- 0301 basic medicine
General Physics and Astronomy
PROTEIN
02 engineering and technology
medicine.disease_cause
Bacteriocins
Gene cluster
Disulfides
Cloning, Molecular
lcsh:Science
Multidisciplinary
biology
Chemistry
Glycopeptides
021001 nanoscience & nanotechnology
Anti-Bacterial Agents
Biochemistry
Multigene Family
SUBLANCIN 168
0210 nano-technology
GENES
Science
Heterologous
Bacterial genome size
Microbial Sensitivity Tests
IMMUNITY
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
Bacteriocin
medicine
Escherichia coli
SPECTRA
Amino Acid Sequence
Bacillaceae
IDENTIFICATION
Sequence Homology, Amino Acid
Thermophile
General Chemistry
biology.organism_classification
Protein Structure, Tertiary
MODEL
030104 developmental biology
Genes, Bacterial
glycocin
glycopeptide
thermophiles
lcsh:Q
Heterologous expression
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....b37eb34fcb4bbc3ef175550f50fde687