Back to Search Start Over

Diagnosis of Intoxication by the Organophosphate VX: Comparison Between an Electrochemical Sensor and Ellman´s Photometric Method

Authors :
Miroslav Pohanka
Martina Hrabinova
Kamil Kuca
Source :
Sensors (Basel, Switzerland), Sensors; Volume 8; Issue 9; Pages: 5229-5237, Sensors, Vol 8, Iss 9, Pp 5229-5237 (2008)
Publication Year :
2008
Publisher :
Molecular Diversity Preservation International (MDPI), 2008.

Abstract

An electrochemical sensor is introduced as a tool applicable for diagnosis of intoxication by cholinesterase inhibitors caused by the well-known nerve agent VX. The traditional Ellman method was chosen for comparison with the sensor's analytical parameters. Both methods are based on estimation of blood cholinesterase inhibition as a marker of intoxication. While Ellman´s method provided a limit of detection of 5.2´10-7 M for blood containing VX, the electrochemical sensor was able to detect 4.0´10-7 M. Good correlation between both methods was observed (R = 0.92). The electrochemical sensor could be considered a convenient tool for a fast yet accurate method, easily available for field as well as laboratory use. Time and cost savings are key features of the sensor-based assay.

Details

Language :
English
ISSN :
14248220
Volume :
8
Issue :
9
Database :
OpenAIRE
Journal :
Sensors (Basel, Switzerland)
Accession number :
edsair.doi.dedup.....b34aae389ee9d0c8d0ab033d6f8a9f6b