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Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast
- Source :
- Toxicological Sciences
- Publication Year :
- 2018
-
Abstract
- To identify target genes against silver nanoparticles (AgNPs), we screened a genome-wide gene deletion library of 4843 fission yeast heterozygous mutants covering 96% of all protein encoding genes. A total of 33 targets were identified by a microarray and subsequent individual confirmation. The target pattern of AgNPs was more similar to those of AgNO3 and H2O2, followed by Cd and As. The toxic effect of AgNPs on fission yeast was attributed to the intracellular uptake of AgNPs, followed by the subsequent release of Ag+, leading to the generation of reactive oxygen species (ROS). Next, we focused on the top 10 sensitive targets for further studies. As described previously, 7 nonessential targets were associated with detoxification of ROS, because their heterozygous mutants showed elevated ROS levels. Three novel essential targets were related to folate metabolism or cellular component organization, resulting in cell cycle arrest and no induction in the transcriptional level of antioxidant enzymes such as Sod1 and Gpx1 when 1 of the 2 copies was deleted. Intriguingly, met9 played a key role in combating AgNP-induced ROS generation via NADPH production and was also conserved in a human cell line.
- Subjects :
- 0301 basic medicine
Heterozygote
silver nanoparticles
Silver
Mutant
Metal Nanoparticles
010501 environmental sciences
Toxicology
01 natural sciences
Antioxidants
03 medical and health sciences
Schizosaccharomyces
NADPH
Genomic library
Gene
0105 earth and related environmental sciences
Gene Library
chemistry.chemical_classification
Reactive oxygen species
biology
met9
Chemistry
Gwas of Silver Nanoparticle Effects in Fission Yeast
ROS
Cell Cycle Checkpoints
biology.organism_classification
fission yeast
Yeast
Cell biology
Cellular component organization
systematic screening
030104 developmental biology
Schizosaccharomyces pombe Proteins
Reactive Oxygen Species
Intracellular
Gene Deletion
Genome-Wide Association Study
Subjects
Details
- ISSN :
- 10960929
- Volume :
- 161
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Toxicological sciences : an official journal of the Society of Toxicology
- Accession number :
- edsair.doi.dedup.....b342900bf527c44cec1582840d06ca7f