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Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch
Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch
- Source :
- Journal of Ethnopharmacology. 118:59-64
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Water-soluble polysaccharide(Glycyrrhiza polysaccharide, GP) was isolated from Glycyrrhiza uralensis Fisch, with glycosidic units were composed of alpha (1-4) linked D-glucana. We demonstrated that GP significantly induces nitric oxides (NO) production and inducible NO synthase (iNOS) transcription in peritoneal macrophages. Moreover, iNOS mRNA expression was strongly induced by GP. NO in the culture supernatant was measured by Griess reaction, the production of iNOS was determined by commercially available iNOS kit. GP (10-400 microg/ml) alone increased significantly NO and iNOS production in macrophages. Macrophages simultaneously treated with GP plus lipopolysaccharide (LPS)/interferon-gamma (IFN-gamma) increased NO and iNOS production as compared to that of GP treatments alone. The production of NO and iNOS in macrophages pretreated with LPS followed by GP was higher than that of treatment with GP and LPS simultaneously. Using RT-PCR reveals that GP may provide a second triggering signal for the expression of iNOS mRNA. Thus, the iNOS-mediated NO synthesis in response to GP may be one of the mechanisms whereby this herbal medicine elicits its therapeutic effects.
- Subjects :
- Lipopolysaccharides
Male
Lipopolysaccharide
Nitric Oxide Synthase Type II
Nitric Oxide
Polysaccharide
Plant Roots
Gene Expression Regulation, Enzymologic
Nitric oxide
Interferon-gamma
Mice
chemistry.chemical_compound
Polysaccharides
Griess test
Drug Discovery
Animals
Glycyrrhiza uralensis
RNA, Messenger
Pharmacology
chemistry.chemical_classification
Mice, Inbred BALB C
Dose-Response Relationship, Drug
biology
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
biology.organism_classification
Molecular biology
Nitric oxide synthase
Enzyme
Biochemistry
Macrophages, Peritoneal
biology.protein
Glycyrrhiza
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....b3413bc0a6b32820d9964d91e4945eb1
- Full Text :
- https://doi.org/10.1016/j.jep.2008.03.002