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PCR for Detection and Identification of Abiotrophia spp
- Source :
- Journal of Clinical Microbiology. 36:2844-2846
- Publication Year :
- 1998
- Publisher :
- American Society for Microbiology, 1998.
-
Abstract
- Members of the genus Abiotrophia , formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiotrophia spp. were developed. The first PCR assay was positive for all Abiotrophia spp. Subsequently performed restriction fragment length polymorphism analysis allowed the verification of the PCR amplicons and the differentiation of the three species. The second PCR assay was positive only for A. elegans , the most fastidious species of Abiotrophia . Both PCR assays were shown to be specific and sensitive and should facilitate the identification of Abiotrophia spp.
- Subjects :
- Microbiology (medical)
Fastidious organism
Molecular Sequence Data
Bacteremia
Biology
medicine.disease_cause
Polymerase Chain Reaction
Sensitivity and Specificity
law.invention
law
RNA, Ribosomal, 16S
medicine
Humans
Ribosomal DNA
Polymerase chain reaction
DNA Primers
Genetics
Base Sequence
Streptococcus
Bacteriology
Endocarditis, Bacterial
Abiotrophia
Amplicon
16S ribosomal RNA
biology.organism_classification
Restriction fragment length polymorphism
Polymorphism, Restriction Fragment Length
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....b32bdc9a776076d1881945c58a38cdf1