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Mixed actions of TMB-8 as a Ca2+ antagonist in cultured mouse cortical neurones
- Source :
- Neuroreport. 5(5)
- Publication Year :
- 1994
-
Abstract
- The action of TMB-8 [8-N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate] on glutamate (Glu)- and 55 mM KCl (K+)-induced increases in intracellular free calcium levels ([Ca2+]i) was studied in cultured mouse cerebral cortical neurones. Glu-induced responses were mediated by two mechanisms, one independent of and the other dependent on extracellular Ca2+, both being inhibited by TMB-8 in a dose-dependent manner. TMB-8 had no effect on [3H]dizocilpine binding kinetics in cortical membrane preparations. The transient and sustained phases of K(+)-induced increases in [Ca2+]i were also inhibited by TMB-8 in a dose-dependent manner. The Ca2+ channel antagonist, verapamil, blocked K(+)-induced responses but had no effect on Glu-induced responses. Although TMB-8 displays multiple effects, its action at voltage-gated Ca2+ channels does not appear to contribute significantly to its inhibition of the Glu-induced increase in [Ca2+]i.
- Subjects :
- Intracellular Fluid
Fluorescence spectrometry
chemistry.chemical_element
Glutamic Acid
Calcium
Biology
Receptors, N-Methyl-D-Aspartate
Membrane Potentials
Potassium Chloride
Mice
Glutamates
Gallic Acid
medicine
Extracellular
Animals
Cerebral Cortex
Neurons
General Neuroscience
Glutamate receptor
Antagonist
Depolarization
Calcium Channel Blockers
Cell Compartmentation
Dizocilpine
chemistry
Verapamil
Biophysics
Dizocilpine Maleate
Neuroscience
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 09594965
- Volume :
- 5
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Neuroreport
- Accession number :
- edsair.doi.dedup.....b329c3d01ccd4479975355c74362912f