Unravelling the biodegradation performance and mechanisms of acid orange 7 by aerobic granular sludge at different salinity levels

Azo dyes wastewater is characterized by high-salinity, however, the biodegradation performance and mechanisms of azo dyes by aerobic granular sludge (AGS) under different salinity levels are still unclear. Herein, the results showed that the reactor performance was almost unaffected at low-salinity levels (0.5%-1.0% salinity), and the removal efficiency of acid orange 7 (AO7) was increased by 2.6%-19.1%, possibly due to the excessive secretion of extracellular polymeric substances (EPS) and the enrichment of functional bacteria. Nevertheless, the microbial cell viability was negatively affected by high-salinity level (2.0% salinity), leading to the deterioration of AO7 and nutrient removal efficiencies. The AO7 removal was achieved by rapid adsorption and slow biodegradation. The biodegradation pathway indicated that AO7 was gradually mineralized in the AGS system through desulfurization, deamination, decarboxylation and hydroxylation. Altogether, this work provides an important reference for the application of AGS technology for treating saline azo dye wastewaters.