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Lichen-forming fungusCaloplaca flavoruscensinhibits transcription factors and chromatin remodeling system in fungi
- Source :
- FEMS Microbiology Letters. 363:fnw113
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Lichen-forming fungi and extracts derived from them have been used as alternative medicine sources for millennia and recently there has been a renewed interest in their known bioactive properties for anticancer agents, cosmetics and antibiotics. Although lichen-forming fungus-derived compounds are biologically and commercially valuable, few studies have been performed to determine their modes of action. This study used chemical-genetic and chemogenomic high-throughput analyses to gain insight into the modes of action of Caloplaca flavoruscens extracts. High-throughput screening of 575 lichen extracts was performed and 39 extracts were identified which inhibited yeast growth. A C. flavoruscens extract was selected as a promising antifungal and was subjected to genome-wide haploinsufficiency profiling and homozygous profiling assays. These screens revealed that yeast deletion strains lacking Rsc8, Pro1 and Toa2 were sensitive to three concentrations (IC25.5, IC25 and IC50, respectively) of C. flavoruscens extract. Gene-enrichment analysis of the data showed that C. flavoruscens extracts appear to perturb transcription and chromatin remodeling.
- Subjects :
- 0301 basic medicine
Antifungal Agents
Lichens
Haploinsufficiency
Fungus
Microbiology
Caloplaca
Chromatin remodeling
Saccharomyces
03 medical and health sciences
Ascomycota
Transcription (biology)
Botany
Genetics
Molecular Biology
Transcription factor
Gene
biology
Homozygote
Chromatin Assembly and Disassembly
biology.organism_classification
Yeast
Chromatin
030104 developmental biology
Biochemistry
Transcription Factors
Subjects
Details
- ISSN :
- 15746968
- Volume :
- 363
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters
- Accession number :
- edsair.doi.dedup.....b314dfdd1f3809a884a389dc6ad725d3