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Flow cytometric analysis of the binding of eleven lectins to human T- and B-cells and to human T- and B-cell lines
- Source :
- Cytometry. 5:204-209
- Publication Year :
- 1984
- Publisher :
- Wiley, 1984.
-
Abstract
- The relative surface binding of 11 lectins to human peripheral blood T- and B-lymphocytes, to Molt-4 and JM T-cell lines, and to 6410 and NC37 B-cell lines was determined by flow cytometry. The lectins from Lens culinaris (LCA), Ricinus communis (RCA), Arachis hypogaea (PNA), Abrus precatorius (APA), Ulex europaeus (UEA-F), Sarothamnus scoparius (SAS-F), Helix pomatia (HPA), Phaseolus coccineus (L-PHA), Glycine max (SBA), and Triticum vulgare (WGA) were fluoresceinated and incubated with living, formaldehyde-fixed, or neuraminidase-treated cells. Except LCA, which preferentially bound to the two B-cell lines tested in this study, none of the other lectins exhibited selective binding to the undifferentiated cells of the cell lines. The T-cell lines and, in part, the peripheral blood T-cells bound less WGA, APA, LCA, and L-PHA than the B-cell lines and the peripheral blood B-cells. Binding of PNA was found only after neuraminidase treatment of the cells; the binding of PNA, HPA, and UEA-F after neuraminidase treatment was higher for the T-cells than the B-cells from peripheral blood. No significant differences were detected between both cell types for RCA, ConA, SBA, and SAS-F.
- Subjects :
- T-Lymphocytes
Biophysics
Cell Line
Pathology and Forensic Medicine
Flow cytometry
Blood cell
Endocrinology
Lectins
Abrus precatorius
medicine
Humans
B cell
B-Lymphocytes
biology
medicine.diagnostic_test
Lectin
Cell Biology
Hematology
Flow Cytometry
biology.organism_classification
Ulex europaeus
medicine.anatomical_structure
Biochemistry
Cell culture
Receptors, Mitogen
biology.protein
Neuraminidase
Subjects
Details
- ISSN :
- 10970320 and 01964763
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Cytometry
- Accession number :
- edsair.doi.dedup.....b306133b0d65c725ff77dcf3507ca264