Back to Search
Start Over
Transcriptome analysis of Ginkgo biloba kernels
- Source :
- Frontiers in Plant Science, Frontiers in Plant Science, Vol 6 (2015)
- Publication Year :
- 2015
- Publisher :
- Frontiers Media S.A., 2015.
-
Abstract
- Ginkgo biloba is a dioecious species native to China with medicinally and phylogenetically important characteristics; however, genomic resources for this species are limited. In this study, we performed the first transcriptome sequencing for Ginkgo kernels at five time points using Illumina paired-end sequencing. Approximately 25.08-Gb clean reads were obtained, and 68,547 unigenes with an average length of 870 bp were generated by de novo assembly. Of these unigenes, 29,987 (43.74%) were annotated in publicly available plant protein database. A total of 3869 genes were identified as significantly differentially expressed, and enrichment analysis was conducted at different time points. Furthermore, metabolic pathway analysis revealed that 66 unigenes were responsible for terpenoid backbone biosynthesis, with up to 12 up-regulated unigenes involved in the biosynthesis of ginkgolide and bilobalide. Differentially expressed genes analysis together with real-time PCR experiments indicated that the synthesis of bilobalide may have interfered with the ginkgolide synthesis process in the kernel. These data can remarkably expand the existing transcriptome resources of Ginkgo, and provide a valuable platform to reveal more on developmental and metabolic mechanisms of this species.
- Subjects :
- Ginkgo biloba
Ginkgo
differentially expressed genes (DEGs)
Sequence assembly
Computational biology
Plant Science
lcsh:Plant culture
Biology
biology.organism_classification
terpenoid backbone biosynthesis
Transcriptome
chemistry.chemical_compound
Bilobalide
chemistry
Plant protein
kernel
Botany
Ginkgolide
lcsh:SB1-1110
tran
Gene
transcriptome
Original Research
Subjects
Details
- Language :
- English
- ISSN :
- 1664462X
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....b2ec08a1305109eaec29f914ca433c0a