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Minority resistant variants are also present in HIV-2-infected antiretroviral-naive patients
- Source :
- Journal of Antimicrobial Chemotherapy. 73:1173-1176
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Objectives To assess the prevalence of minority resistant variants (MRV) and X4-tropic minority variants in ART-naive HIV-2-infected patients. Patients and methods ART-naive HIV-2-infected patients with detectable plasma viral load (>100 copies/mL) included in the ANRS HIV-2 CO5 Cohort were assessed. We performed ultra-deep sequencing (UDS) of protease, RT, integrase and gp105 regions. Only mutations in the HIV-2 ANRS list >1% were considered. HIV-2 tropism was assessed by V3 loop region UDS, and each read was interpreted with determinants of CXCR4-coreceptor use. Results Among the 47 patients assessed, three displayed plasma viruses with a resistance-associated mutation (RAM) above the 20% detection threshold, all in RT, resulting in a prevalence of transmitted drug resistance for NRTI of 7.9% (95% CI 0.0%-16.5%). No RAM above the 20% detection threshold was found in protease or integrase. At the 1% detection threshold the transmitted drug resistance prevalence was 9.8% (95% CI 0.6%-19.0%), 13.2% (95% CI 3.5%-22.9%) and 4.5% (95% CI 0%-17.5%) for PI, NRTI and integrase inhibitors. The most prevalent MRV was the PI RAM I50V detected in three samples. Tropism analysis showed that 21% of patients (4 of 19) exhibited X4-tropic viruses: two in majority proportion and two in minority proportions (1.5% and 1.9%). Conclusions In this first study assessing the prevalence of MRV in HIV-2 infection among ART-naive patients, we observed a 2-3-fold higher prevalence of RAM when a 1% detection threshold of mutations was used compared with a 20% threshold. Similarly, the proportion of patients with X4-tropic viruses was twice as high when UDS was used.
- Subjects :
- Adult
Male
0301 basic medicine
Microbiology (medical)
Genotype
030106 microbiology
Integrase inhibitor
HIV Infections
Drug resistance
Cohort Studies
Viral Proteins
03 medical and health sciences
Gene Frequency
Drug Resistance, Viral
Prevalence
Humans
Medicine
Pharmacology (medical)
Tropism
Pharmacology
biology
business.industry
High-Throughput Nucleotide Sequencing
Middle Aged
Viral Load
Virology
Integrase
Infectious Diseases
HIV-2
Mutation
Cohort
biology.protein
Female
business
Viral load
Cohort study
Subjects
Details
- ISSN :
- 14602091 and 03057453
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....b2d41d54fea0285bde1967bdacd03457