A genetically encoded sensor for measuring serotonin dynamics

Authors :: Mimi Shin
Xuelin Li
J. Julius Zhu
Peng Zhang
Jiesi Feng
Wanling Peng
Suyu Hao
Miao Jing
Jing Zou
B. Jill Venton
Tongrui Qian
Jianzhi Zeng
Fei Deng
Yulong Li
Sunlei Pan
Jinxia Wan
Kun Song
Min Xu
Yajun Zhang
Source :: Nature neuroscience
Publication Year :: 2021
Publisher :: Springer Science and Business Media LLC, 2021.
Abstract: Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.