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Prevalence of Campylobacter Species in Adult Crohn's Disease and the Preferential Colonization Sites of Campylobacter Species in the Human Intestine
- Source :
- PLoS ONE, PLoS ONE, Vol 6, Iss 9, p e25417 (2011)
- Publication Year :
- 2011
- Publisher :
- Public Library of Science, 2011.
-
Abstract
- IntroductionCrohn's disease (CD) and ulcerative colitis (UC) are the two major forms of inflammatory bowel disease (IBD). A high prevalence of Campylobacter concisus was previously detected in paediatric CD and adult UC. Currently, the prevalence of C. concisus in adult CD and the preferential colonization sites of Campylobacter species in the human intestine are unknown. In this study, we examined the prevalence of Campylobacter species in biopsies collected from multiple anatomic sites of adult patients with IBD and controls.MethodsThree hundred and one biopsies collected from ileum, caecum, descending colon and rectum of 28 patients IBD (15 CD and 13 UC) and 33 controls were studied. Biopsies were used for DNA extraction and detection of Campylobacter species by PCR-sequencing and Campylobacter cultivation.ResultsA significantly higher prevalence of C. concisus in colonic biopsies of patients with CD (53%) was detected as compared with the controls (18%). Campylobacter genus-PCR positivity and C. concisus positivity in patients with UC were 85% and 77% respectively, being significantly higher than that in the controls (48% and 36%). C. concisus was more often detected in descending colonic and rectal biopsies from patients with IBD in comparison to the controls. C. concisus was isolated from patients with IBD.ConclusionThe high intestinal prevalence of C. concisus in patients with IBD, particularly in the proximal large intestine, suggests that future studies are needed to investigate the possible involvement of C. concisus in a subgroup of human IBD. To our knowledge, this is the first report of the association between adult CD and C. concisus as well as the first study of the preferential colonization sites of C. concisus in the human intestine.
- Subjects :
- Bacterial Diseases
Male
Biopsy
medicine.disease_cause
Gastroenterology
Inflammatory bowel disease
Polymerase Chain Reaction
Crohn Disease
Gram Negative
Large intestine
Gastrointestinal Infections
Crohn's disease
Multidisciplinary
Campylobacter
Middle Aged
Ulcerative colitis
Bacterial Pathogens
Host-Pathogen Interaction
Intestines
medicine.anatomical_structure
Infectious Diseases
Medicine
Female
Research Article
Adult
medicine.medical_specialty
Colon
Science
Campylobacter concisus
Ileum
Gastroenterology and Hepatology
Biology
Microbiology
Descending colon
Microbial Ecology
Internal medicine
medicine
Ulcerative Colitis
Humans
Inflammation
Inflammatory Bowel Disease
Immunity
Rectum
biology.organism_classification
medicine.disease
digestive system diseases
Emerging Infectious Diseases
Immunology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....b2baf2bf455f52c261396b19a061ceac