Morphological analysis of cementum and root dentin after Er:YAG laser irradiation

Background and Objectives To investigate the morphology of cementum and root dentin after Er:YAG laser irradiation with and without water coolant, compared to that after CO2 laser irradiation and an untreated surface. Study Design/Materials and Methods Ten extracted healthy human teeth were used. Er:YAG and CO2 lasers were applied with energy outputs of 0.4 W, with and without coolant and 0.5 W, without coolant, respectively. Scanning electron microscopy (SEM) analysis was performed at high and ultra-high magnifications. Results The surface of cementum was micro-irregular with numerous projections while that of dentin appeared scaly after Er:YAG laser irradiation. Unlike after CO2 laser treatment, no major melting or cracking was observed with Er:YAG laser treatment. The use of water spray produced fine micro-irregularities without attached debris. Ultra-high magnification revealed similar microparticles-composed aspects for both cementum and dentin. However, the more porous structure of the surface was observed after Er:YAG laser irradiation without water spray. Conclusions Cementum and root dentin presented distinct micro-roughness after Er:YAG laser irradiation, possibly due to structural differences in the original tissue. However, under ultra-high magnifications, both cementum and dentin presented similar characteristics of the irradiated surface. In addition, the use of water spray during laser irradiation minimized thermal effects and resulted in a cleaner and less porous surface. Lasers Surg. Med. 31:79–85, 2002. © 2002 Wiley-Liss, Inc.