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Automated Patch Clamp Analysis of nAChα7 and Na(V)1.7 Channels
- Source :
- Current Protocols in Pharmacology
- Publication Year :
- 2014
-
Abstract
- Automated patch clamp devices are now commonly used for studying ion channels. A useful modification of this approach is the replacement of the glass pipet with a thin planar glass layer with a small hole in the middle. Planar patch clamp devices, such as the three described in this unit, are overtaking glass pipets in popularity because they increase throughput, are easier to use, provide for the acquisition of high-quality and information-rich data, and allow for rapid perfusion and temperature control. Covered in this unit are two challenging targets in drug discovery: voltage-gated sodium subtype 1.7 (NaV1.7) and nicotinic acetylcholine α7 receptors (nAChα7R). Provided herein are protocols for recording activation and inactivation kinetics of NaV1.7, and activation and allosteric modulation of nAChα7R. Curr. Protoc. Pharmacol. 65:11.13.1-11.13.48. © 2014 by John Wiley & Sons, Inc. Keywords: automated patch clamp; NaV1.7; nicotinic acetylcholine receptors
- Subjects :
- Automation, Laboratory
Patch-Clamp Techniques
alpha7 Nicotinic Acetylcholine Receptor
Drug discovery
Chemistry
NAV1.7 Voltage-Gated Sodium Channel
Pipette
Analytical chemistry
General Medicine
CHO Cells
Nicotinic agonist
Cricetulus
HEK293 Cells
Automated patch clamp
medicine
Animals
Humans
Patch clamp
Acetylcholine
Ion channel
medicine.drug
Biomedical engineering
Acetylcholine receptor
Subjects
Details
- ISSN :
- 19348290
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Current protocols in pharmacology
- Accession number :
- edsair.doi.dedup.....b29c4c2fe3c08b15dba3350dcaaa9233