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β-lactam antibiotics promote bacterial mutagenesis via an RpoS-mediated reduction in replication fidelity
- Source :
- Nature Communications, Nature Communications, 2013, 4, pp.1610. ⟨10.1038/ncomms2607⟩, Nature Communications, Nature Publishing Group, 2013, 4, pp.1610. ⟨10.1038/ncomms2607⟩, Nature Communications; Vol 4
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Antimicrobials: mismatch excels when ampicillin runs low. [Nat Rev Microbiol. 2013]; International audience; Regardless of their targets and modes of action, subinhibitory concentrations of antibiotics can have an impact on cell physiology and trigger a large variety of cellular responses in different bacterial species. Subinhibitory concentrations of β-lactam antibiotics cause reactive oxygen species production and induce PolIV-dependent mutagenesis in Escherichia coli. Here we show that subinhibitory concentrations of β-lactam antibiotics induce the RpoS regulon. RpoS regulon induction is required for PolIV-dependent mutagenesis because it diminishes the control of DNA-replication fidelity by depleting MutS in E. coli, Vibrio cholerae and Pseudomonas aeruginosa. We also show that in E. coli, the reduction in mismatch-repair activity is mediated by SdsR, the RpoS-controlled small RNA. In summary, we show that mutagenesis induced by subinhibitory concentrations of antibiotics is a genetically controlled process. Because this mutagenesis can generate mutations conferring antibiotic resistance, it should be taken into consideration for the development of more efficient antimicrobial therapeutic strategies.
- Subjects :
- DNA Replication
medicine.drug_class
[SDV]Life Sciences [q-bio]
Antibiotics
General Physics and Astronomy
Sigma Factor
MESH: DNA Replication
Biology
beta-Lactams
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
Microbiology
03 medical and health sciences
Antibiotic resistance
Bacterial Proteins
MESH: beta-Lactams
MESH: Anti-Bacterial Agents
Escherichia coli
medicine
Vibrio cholerae
MESH: Bacterial Proteins
030304 developmental biology
MESH: Mutagenesis
0303 health sciences
Multidisciplinary
Bacteria
030306 microbiology
Pseudomonas aeruginosa
MESH: Escherichia coli
Mutagenesis
MESH: Reactive Oxygen Species
MESH: Sigma Factor
General Chemistry
biochemical phenomena, metabolism, and nutrition
Anti-Bacterial Agents
3. Good health
[SDV] Life Sciences [q-bio]
MESH: Bacteria
Regulon
MESH: Pseudomonas aeruginosa
bacteria
Reactive Oxygen Species
rpoS
MESH: Vibrio cholerae
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, 2013, 4, pp.1610. ⟨10.1038/ncomms2607⟩, Nature Communications, Nature Publishing Group, 2013, 4, pp.1610. ⟨10.1038/ncomms2607⟩, Nature Communications; Vol 4
- Accession number :
- edsair.doi.dedup.....b29287eaf75f833884d9f1b7fdde67fb