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Novel herpesviruses in neotropical bats and their relationship with other members of the Herpesviridae family
- Source :
- Infection, Genetics and Evolution, Infection, Genetics and Evolution, 2020, 84, pp.104367. ⟨10.1016/j.meegid.2020.104367⟩, Infection, Genetics and Evolution, Elsevier, 2020, pp.104367. ⟨10.1016/j.meegid.2020.104367⟩
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- In the past decade, a large number of studies have detected herpesvirus sequences from many bat species around the world. Nevertheless, the discovery of bat herpesviruses is geographically uneven. Of the various bat species tested to date, only a few were from the New World. Seeking to investigate the distribution and diversity of herpesviruses circulating in neotropical bats, we carried out molecular screening of 195 blood DNA samples from 11 species of three bat families (Phyllostomidae, Mormoopidae, and Molossidae). Using polymerase chain reaction amplification, with degenerate consensus primers targeting highly conserved amino acid motifs of the herpesvirus DNA polymerase and Glycoprotein B genes, we characterized novel viral sequences from all tested species. BLAST searches, pairwise nucleotide and amino acid sequence comparisons, as well as phylogenetic analyses confirmed that they all belonged to the Herpesviridae family, of the Beta- and Gammaherpesvirinae subfamilies. Fourteen partial DNA polymerase gene sequences, of which three beta- and 11 gamma-herpesviruses, were detected. A total of 12 partial Glycoprotein B gene sequences, all gamma-herpesviruses, were characterized. Every sequence was specific to a bat species and in some species (Desmodus rotundus, Carollia perspicillata, and Pteronotus rubiginosus) multiple viruses were found. Phylogenetic analyses of beta- and gammaherpesvirus sequences led to the identification of bat-specific clades. Those composed of sequences obtained from different bat species belonging to distinct subfamilies follow the taxonomy of bats. This study confirms the astonishing diversity of bat herpesviruses and broadens our knowledge of their host range. Nevertheless, it also emphasizes the fact that, to better appreciate the evolutionary history of these viruses, much remains to be done at various taxonomic levels.<br />Highlights • Molecular screening was carried out on 11 bat species from French Guiana and Martinique (French West Indies). • Partial DNA polymerase gene sequences of 14 viruses were characterized, three beta- and 11 gammaherpesviruses, as well as 12 Glycoprotein B sequences, all of gamma-herpesviruses. • Genetic characterization of these sequences reveals a high degree of genetic divergence. • On a phylogenetic viewpoint, most of the newly discovered herpesvirus sequences fall within bat-specific clades well correlated with the taxonomy of their hosts. • This study is the largest conducted to date in terms of species diversity from the New World.
- Subjects :
- 0301 basic medicine
[SDV]Life Sciences [q-bio]
DNA-Directed DNA Polymerase
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy
Mormoopidae
law.invention
law
Chiroptera
Gammaherpesvirinae
Molossidae
Herpesviridae
Phylogeny
Polymerase chain reaction
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Carollia perspicillata
biology
Phylogenetic tree
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
Betaherpesvirus
French Guiana
3. Good health
[SDV] Life Sciences [q-bio]
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Infectious Diseases
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Glycoprotein B
Microbiology (medical)
Evolution
030106 microbiology
[SDV.BID]Life Sciences [q-bio]/Biodiversity
DNA polymerase
Microbiology
Article
Gammaherpesvirus
Viral Proteins
03 medical and health sciences
Phylogenetics
[SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy
[SDV.BID.EVO] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
Genetics
Animals
Martinique
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Glycoproteins
biology.organism_classification
Exodeoxyribonucleases
030104 developmental biology
Evolutionary biology
Desmodus rotundus
[SDV.BID] Life Sciences [q-bio]/Biodiversity
Subjects
Details
- ISSN :
- 15671348 and 15677257
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Infection, Genetics and Evolution
- Accession number :
- edsair.doi.dedup.....b2806b34d35ae6949959af0c95799563
- Full Text :
- https://doi.org/10.1016/j.meegid.2020.104367