Back to Search
Start Over
A Generic LC-HRMS Screening Method for Marine and Freshwater Phycotoxins in Fish, Shellfish, Water, and Supplements
- Source :
- Toxins; Volume 13; Issue 11; Pages: 823, Toxins, 13(11), Toxins, Toxins, Vol 13, Iss 823, p 823 (2021), Toxins 13 (2021) 11
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Phycotoxins occur in various marine and freshwater environments, and can accumulate in edible species such as fish, crabs, and shellfish. Human exposure to these toxins can take place, for instance, through consumption of contaminated species or supplements and through the ingestion of contaminated water. Symptoms of phycotoxin intoxication include paralysis, diarrhea, and amnesia. When the cause of an intoxication cannot directly be found, a screening method is required to identify the causative toxin. In this work, such a screening method was developed and validated for marine and freshwater phycotoxins in different matrices: fish, shellfish, water, and food supplements. Two LC methods were developed: one for hydrophilic and one for lipophilic phycotoxins. Sample extracts were measured in full scan mode with an Orbitrap high resolution mass spectrometer. Additionally, a database was created to process the data. The method was successfully validated for most matrices, and in addition, regulated lipophilic phycotoxins, domoic acid, and some paralytic shellfish poisoning toxins could be quantified in shellfish. The method showed limitations for hydrophilic phycotoxins in sea water and for lipophilic phycotoxins in food supplements. The developed method is a screening method; in order to confirm suspected compounds, comparison with a standard or an additional analysis such as NMR is required.
- Subjects :
- Project- en Accountmanagement
Health, Toxicology and Mutagenesis
Programme and Account Management
Fresh Water
Toxicology
High resolution mass spectrometry
Mass Spectrometry
Article
chemistry.chemical_compound
Food supplements
Screening method
medicine
Ingestion
Animals
Food science
Paralytic shellfish poisoning
HILIC
Shellfish
Phycotoxins
Reversed phase
Phycotoxin
Kainic Acid
Domoic acid
Water
food and beverages
medicine.disease
phycotoxins
screening
high resolution mass spectrometry
reversed phase
shellfish
water
food supplements
Team Natural Toxins
chemistry
Seafood
Human exposure
Dietary Supplements
Screening
Fish
Medicine
Marine Toxins
Hydrophobic and Hydrophilic Interactions
Chromatography, Liquid
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Database :
- OpenAIRE
- Journal :
- Toxins; Volume 13; Issue 11; Pages: 823
- Accession number :
- edsair.doi.dedup.....b26963a9d67024908abc1eaee1a452a6
- Full Text :
- https://doi.org/10.3390/toxins13110823