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Role of TLR4/MyD88/NF-κB signaling in heart and liver-related complications in a rat model of type 2 diabetes mellitus
- Source :
- The Journal of International Medical Research, Journal of International Medical Research, Vol 49 (2021)
- Publication Year :
- 2021
-
Abstract
- Aims To analyze expression of members of the Toll-like receptor (TLR)4/myeloid differentiation primary response 88 (MyD88)/nuclear factor (NF)-κB signaling pathway in the heart and liver in a rat model of type 2 diabetes mellitus (T2DM). Our overall goal was to understand the underlying pathophysiological mechanisms. Methods We measured fasting blood glucose (FBG) and insulin (FINS) in a rat model of T2DM. Expression of members of the TLR4/MyD88/NF-κB signaling pathway as well as downstream cytokines was investigated. Levels of mRNA and protein were assessed using quantitative real-time polymerase chain reaction and western blotting, respectively. Protein content of tissue homogenates was assessed using enzyme-linked immunosorbent assays. Results Diabetic rats had lower body weights, higher FBG, higher FINS, and higher intraperitoneal glucose tolerance than normal rats. In addition, biochemical indicators related to heart and liver function were elevated in diabetic rats compared with normal rats. TLR4 and MyD88 were involved in the occurrence of T2DM as well as T2DM-related heart and liver complications. TLR4 caused T2DM-related heart and liver complications through activation of NF-κB. Conclusions TLR4/MyD88/NF-κB signaling induces production of tumor necrosis factor-α, interleukin-6, and monocyte chemoattractant protein-1, leading to the heart- and liver-related complications of T2DM.
- Subjects :
- 0301 basic medicine
Medicine (General)
Prospective Clinical Research Report
Rat model
nuclear factor-κB
heart
liver
Biochemistry
Myeloid differentiation primary response
Diabetes Mellitus, Experimental
03 medical and health sciences
0302 clinical medicine
Insulin resistance
R5-920
insulin resistance
Type 2 diabetes mellitus
medicine
Animals
Receptor
business.industry
Biochemistry (medical)
NF-kappa B
Type 2 Diabetes Mellitus
Cell Biology
General Medicine
medicine.disease
Toll-like receptor 4
Rats
Nf κb signaling
030104 developmental biology
Diabetes Mellitus, Type 2
030220 oncology & carcinogenesis
Myeloid Differentiation Factor 88
Cancer research
TLR4
Signal transduction
tumor necrosis factor-α
business
Signal Transduction
Subjects
Details
- ISSN :
- 14732300
- Volume :
- 49
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Journal of international medical research
- Accession number :
- edsair.doi.dedup.....b25a238df00e10f320af69f3736ad936