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A novel two-layer-integrated microfluidic device for high-throughput yeast proteomic dynamics analysis at the single-cell level
- Source :
- Integrative Biology. 12:241-249
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Current microfluidic methods for studying multicell strains (e.g., m-types) with multienvironments (e.g., n-types) require large numbers of inlets/outlets (m*n), a complicated procedure or expensive machinery. Here, we developed a novel two-layer-integrated method to combine different PDMS microchannel layers with different functions into one chip by a PDMS through-hole array, which improved the design of a PDMS-based microfluidic system. Using this method, we succeeded in converting 2 × m × n inlets/outlets into m + n inlets/outlets and reduced the time cost of loading processing (from m × n to m) of the device for studying multicell strains (e.g., m-types) in varied multitemporal environments (i.e., n-types). Using this device, the dynamic behavior of the cell-stress-response proteins was studied when the glucose concentration decreased from 2% to a series of lower concentrations. Our device could also be widely used in high-throughput studies of various stress responses, and the new concept of a multilayer-integrated fabrication method could greatly improve the design of PDMS-based microfluidic systems.
- Subjects :
- Proteomics
Materials science
Fabrication
Proteome
Green Fluorescent Proteins
Microfluidics
Active Transport, Cell Nucleus
Biophysics
Two layer
Saccharomyces cerevisiae
Cellular level
Biochemistry
Lab-On-A-Chip Devices
Image Processing, Computer-Assisted
Dimethylpolysiloxanes
Throughput (business)
Cell Nucleus
Microchannel
business.industry
Equipment Design
Chip
Yeast
Protein Transport
Glucose
Optoelectronics
business
Transcription Factors
Subjects
Details
- ISSN :
- 17579708
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Integrative Biology
- Accession number :
- edsair.doi.dedup.....b2379dc597ece50fd13e786396b4a14f