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Single nucleoprotein residue determines influenza A virus sensitivity to an intertypic suppression mechanism
- Source :
- Virology. 506
- Publication Year :
- 2017
-
Abstract
- Several mechanisms underlying intertypic interference between co-infecting influenza types A and B viruses (IAV and IBV) have been proposed. We have recently described one in which IBV's nucleoprotein (BNP) sequestered IAV's nucleoprotein (ANP) and suppressed IAV polymerase and growth. However, its anti-IAV capacity and limitations have not been fully explored. Here, we showed that BNP's inhibitory effect was more potent toward a wide array of avian IAVs, whereas human IAVs revealed moderate resistance. BNP sensitivity was largely determined by ANP's residue 343 at the NP oligomerization interface. An avian IAV polymerase carrying an NP-V343L mutation switched from being highly BNP-sensitive to moderately BNP-resistant, and vice versa for a human IAV polymerase carrying a reverse mutation. To highlight its capacity, we demonstrated that the polymerases of highly-pathogenic H5N1 and the pandemic 2009 (H1N1) strains are strongly inhibited by BNP. Our work provides insights into lineage-specific sensitivity to BNP-mediated intertypic interference.
- Subjects :
- 0301 basic medicine
Biology
medicine.disease_cause
Polymorphism, Single Nucleotide
Birds
03 medical and health sciences
Residue (chemistry)
Influenza A Virus, H1N1 Subtype
Virology
Influenza, Human
Influenza A virus
medicine
Animals
Humans
cardiovascular diseases
Polymerase
Mutation
Influenza A Virus, H5N1 Subtype
Mechanism (biology)
Influenza A Virus, H3N2 Subtype
Reverse mutation
Influenza A virus subtype H5N1
Nucleoprotein
Influenza B virus
030104 developmental biology
Nucleoproteins
Influenza in Birds
cardiovascular system
biology.protein
human activities
hormones, hormone substitutes, and hormone antagonists
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 10960341
- Volume :
- 506
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....b20e5b4873da13ca3e1791f991d329e6