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Holocrine Secretion Occurs outside the Tight Junction Barrier in Multicellular Glands: Lessons from Claudin-1–Deficient Mice
- Source :
- Journal of Investigative Dermatology. 140:298-308.e5
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Holocrine secretion is a specific mode of secretion involving secretion of entire cytoplasmic materials with remnants of dead cells, as observed in multicellular exocrine glands of reptiles, birds, and mammals. Here, we found that sebaceous glands in mice, representative of multicellular exocrine glands of mammals, exhibit a form of polarized stratified epithelium equipped with tight junctions (TJs), and found that holocrine secretion occurred outside the TJ barriers. Sebaceous glands share characteristics of stratified epithelia with interfollicular epidermis, including basal-layer-restricted cell proliferation, TJ barrier formation at a specific single layer of cells with apico-basolateral plasma membrane polarity, and cell death outside the TJ barrier. Knockout of claudin-1, a transmembrane adhesive protein in TJs, in mice caused leakage of the TJ barrier in sebaceous glands and incomplete degradation of the plasma membrane and nuclei during holocrine secretion. Claudin-1 knockout resulted in the accumulation of incompletely degenerated sebocytes in sebaceous ducts, suggesting that the TJ barrier was necessary for differentiation of holocrine secretion. The redefinition of sebaceous glands as TJ-forming stratified epithelia provides an important framework to understand the molecular mechanism of holocrine secretion.
- Subjects :
- 0301 basic medicine
Programmed cell death
Exocrine gland
Cellular differentiation
Dermatology
Biochemistry
Tight Junctions
Mice
Sebaceous Glands
03 medical and health sciences
0302 clinical medicine
Claudin-1
Conditional gene knockout
medicine
Animals
Secretion
Claudin
Molecular Biology
Cells, Cultured
Cell Nucleus
Mice, Knockout
Tight junction
Chemistry
Cell Membrane
Holocrine
Cell Differentiation
Epithelial Cells
Cell Biology
Cell biology
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....b20448dfeb2a1e24583d974eaa4bce96