Real-time multi-analyte online monitoring of 3d cell cultures by integrated enzyme-based biosensors in hanging drop networks

Authors :: Joerg Rothe
Andreas Hierlemann
O. Frey
Patrick M. Misun
Source :: 2015 Transducers-2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)
Publication Year :: 2015
Publisher :: IEEE, 2015.
Abstract: We present the integration of enzyme-based lactate and glucose biosensors into hanging-drop networks. Hangingdrop networks are an ideal microfluidic platform for formation, cultivation, and continuous and long-term observation of 3D microtissues. The implementation of biosensors enables in-situ online monitoring of the effects of different culturing conditions and compound dosages on the microtissues. A hybrid approach including glass sensor modules embedded into a microfluidic polydimethylsiloxane (PDMS)-based chip facilitates system integration. The biosensors enable real-time recording of lactate production and glucose consumption of human colon carcinoma spheroids.<br />2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)<br />ISBN:978-1-4799-8955-3

Subjects :: Hanging-drop network
Materials science
Polydimethylsiloxane
Drop (liquid)
Microfluidics
technology, industry, and agriculture
Nanotechnology
macromolecular substances
Hybrid approach
chemistry.chemical_compound
Glucose
chemistry
Lactate
Biosensor
3D microtissues
Human colon
Multi analyte
Biomedical engineering

ISBN :: 978-1-4799-8955-3
ISBNs :: 9781479989553
Database :: OpenAIRE
Journal :: 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)
Accession number :: edsair.doi.dedup.....b1f6f1f3ab48e25c6304bc364aa2f612
Full Text :: https://doi.org/10.1109/transducers.2015.7181252

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